Record details

Living Modified Organism identity

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LMO name

Moonberry carnation

Transformation event

25958

Unique identifier

IFD-25958-3

Developer(s)

Record #104607

Dr Yukihisa Katsumoto

Principal Researcher

Research Institute

Suntory Holdings Limited (SHD)

1-1-1 Wakayamadai, Shimamoto-cho

Mishima-gun, Osaka

Japan, 618-8503

Phone:	+81 75 962 9132
Fax:	+81 75 962 3791
Email:	Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp

Description

Carnation variety moonberry flowers have a unique violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.

The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.

A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #4954

Dianthus caryophyllus - Carnation, DIACA

Related LMOs

Record #104608

IFD-25947-1 - Moonpearl carnation

Dr Yukihisa Katsumoto Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea

Characteristics of the transformation process

Vector

pCGP3366

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

CaMV 35S promoter

#100287

0.19 Kb

5' untranslated leader of chlorophyll a/b-binding protein

#101901

0.06 Kb

Acetohydroxy acid synthase gene

#15177

1.99 Kb

Acetohydroxy acid synthase gene terminator

#100390

1.77 Kb

Chalcone synthase gene promoter

#103771

1.16 Kb

Flavonoid 3’, 5’-hydroxylase gene

#43793

1.78 Kb

D8 gene terminator

#103772

0.82 Kb

Dihydroflavonol-4-reductase promoter

#105798

2.98 Kb

Dihydroflavonol-4-reductase

#15009

1.70 Kb

Dihydroflavonol-4-reductase terminator

#105799

0.28 Kb

CaMV 35S promoter

#100287

0.42 Kb

Dihydroflavonol-4-reductase

#104594

0.26 Kb

Dihydroflavonol-4-reductase intron

#105819

0.19 Kb

Dihydroflavonol-4-reductase

#104594

0.26 Kb

CaMV 35S terminator

#100290

0.22 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.

The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis

LMO characteristics

Modified traits

Changes in quality and/or metabolite content
- Pigmentation / Coloration
- Flavonoids (e.g. anthocyanin)
Resistance to herbicides
- Chlorsulfuron
- Sulfonylurea

Common use(s)

Ornamental

Additional Information

Moonberry was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing three genes that function together in the biosynthesis of the anthocyanin pigment delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.

Other relevant website address or attached documents

IFD-25958-3 - ISAAA

Carnation Moonberry 25958-3 - GMO Compass