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Modified Organism
IFD-25958-3 - Moonberry carnation
Record information and status
Record ID
104611
Status
Published
Date of creation
2013-01-14 23:03 UTC (schandler@florigene.com.au)
Date of last update
2014-08-28 15:02 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-28 15:02 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonberry carnation
Transformation event
25958
Unique identifier
IFD-25958-3
Developer(s)
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:+81 75 962 9132
Fax:+81 75 962 3791
Email:Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp
Description
Carnation variety moonberry flowers have a unique violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.

The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.

A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Related LMOs
IFD-25947-1 - Moonpearl carnation
Dr Yukihisa Katsumoto Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP3366
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
1.99 Kb
 
 
Acetohydroxy acid synthase gene terminator
1.77 Kb
 
 
Chalcone synthase gene promoter
1.16 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.78 Kb
 
 
D8 gene terminator
0.82 Kb
 
 
Dihydroflavonol-4-reductase promoter
2.98 Kb
 
 
Dihydroflavonol-4-reductase
1.70 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.28 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Dihydroflavonol-4-reductase
0.26 Kb
 
 
Dihydroflavonol-4-reductase intron
0.19 Kb
 
 
Dihydroflavonol-4-reductase
0.26 Kb
 
 
CaMV 35S terminator
0.22 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.

The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Additional Information
Moonberry was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing three genes that function together in the biosynthesis of the anthocyanin pigment delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.
Other relevant website address or attached documents

Records referencing this document (12)
IDDescription
12record(s) found
Country's Decision or any other Communication5 records
Modified Organism2 records
Risk Assessment5 records