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Record details
Modified Organism
IFD-25958-3 - Moonberry carnation
LMO Information
Decisions on the LMO
Risk Assessments
Record information and status
Record ID
104611
Status
Published
Date of creation
2013-01-14 23:03 UTC (schandler@florigene.com.au)
Date of last update
2014-08-28 15:02 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-28 15:02 UTC (dina.abdelhakim@cbd.int)
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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the
LMO quick-links
page.
LMO name
Moonberry carnation
Transformation event
25958
Unique identifier
IFD-25958-3
Developer(s)
Record #104607
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:
+81 75 962 9132
Fax:
+81 75 962 3791
Email:
Yoshikazu_Tanaka@suntory.co.jp
,
Yukihisa_Katsumoto@suntory.co.jp
Description
Carnation variety moonberry flowers have a unique violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.
The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.
A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Record #4954
Dianthus caryophyllus - Carnation, DIACA
Related LMOs
Record #104608
IFD-25947-1 - Moonpearl carnation
Dr Yukihisa Katsumoto Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP3366
Techniques used for the modification
Agrobacterium-mediated DNA transfer
Genetic elements construct
CaMV 35S promoter
#100287
0.19 Kb
5' untranslated leader of chlorophyll a/b-binding protein
#101901
0.06 Kb
Acetohydroxy acid synthase gene
#15177
1.99 Kb
Acetohydroxy acid synthase gene terminator
#100390
1.77 Kb
Chalcone synthase gene promoter
#103771
1.16 Kb
Flavonoid 3’, 5’-hydroxylase gene
#43793
1.78 Kb
D8 gene terminator
#103772
0.82 Kb
Dihydroflavonol-4-reductase promoter
#105798
2.98 Kb
Dihydroflavonol-4-reductase
#15009
1.70 Kb
Dihydroflavonol-4-reductase terminator
#105799
0.28 Kb
CaMV 35S promoter
#100287
0.42 Kb
Dihydroflavonol-4-reductase
#104594
0.26 Kb
Dihydroflavonol-4-reductase intron
#105819
0.19 Kb
Dihydroflavonol-4-reductase
#104594
0.26 Kb
CaMV 35S terminator
#100290
0.22 Kb
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.
The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
LMO characteristics
Modified traits
Changes in quality and/or metabolite content
Pigmentation / Coloration
Flavonoids (e.g. anthocyanin)
Resistance to herbicides
Chlorsulfuron
Sulfonylurea
Common use(s)
Ornamental
Additional Information
Additional Information
Moonberry was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing three genes that function together in the biosynthesis of the anthocyanin pigment delphinidin.
The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family.
Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR.
Other relevant website address or attached documents
IFD-25958-3 - ISAAA
Carnation Moonberry 25958-3 - GMO Compass
Records referencing this document
(
16
)
ID
Description
16
record(s) found
Country's Decision or any other Communication
6 records
Modified Organism
2 records
Risk Assessment
8 records
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