Record details

Living Modified Organism identity

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LMO name

Dicamba Tolerant Soybean

Transformation event

MON87708

Unique identifier

MON-877Ø8-9

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Description

MON 87708 soybean line was developed as a marker-free LMO to exhibit tolerance to dicamba (3,6-dichloro-2-methoxybenzoic acid) herbicide.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #10453

Glycine max - Soybean, Soya bean, Soya, SOYBN

Characteristics of the transformation process

Vector

PV-GMHT4355

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

PC1SV Promoter

#104662

0.43 Kb

TEV 5' Untranslated Region

#104664

0.13 Kb

rbcS Transit Peptide

#103616

0.24 Kb

Dicamba monooxygenase gene

#100728

1.02 Kb

rbcS-E9 gene terminator

#101877

0.64 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The PV-GMHT4355 plasmid contained a second T-DNA cassette that encoded for the glyphosate tolerant form of EPSPS. The cassette contained the following elements in an antisense orientation to the dicamba expression cassette: P-FMV>> L-DnaK>> TS-CTP2>> CS-C4 EPSPS>> T-E9.

Southern blot analysis indicated that this expression cassette was not integrated into the MON87708 line and neither were any elements of the vector backbone.

Southern blot analysis and sequencing analysis indicated that a single intact dicamba expression cassette was integrated into the MON87708 line.

LMO characteristics

Modified traits

Resistance to herbicides

Common use(s)

Food
Feed

Detection method(s)

External link(s)

MON-877Ø8-9 - GMOMETHODS

Additional Information

Other relevant website address or attached documents

Dicamba Tolerant MON 87708- APHIS.pdf

Dicamba Tolerant MON 87708 soybean - FDA