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Modified Organism
DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6 x SYN-IR6Ø4-5 x MON-ØØ6Ø3-6 - Maize modified for resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide
Record information and status
Record ID
104679
Status
Published
Date of creation
2013-02-07 21:40 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-07-24 18:51 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 18:51 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide
Transformation event
TC1507 x DAS59122 x MON810 x MIR604 x NK603
Unique identifier
DAS-Ø15Ø7-1 x DAS-59122-7 x MON-ØØ81Ø-6 x SYN-IR6Ø4-5 x MON-ØØ6Ø3-6
Developer(s)
Du Pont Kabushiki Kaisha
Sanno Park Tower 2-11-1, Nagata-chou
Chiyoda-ku, Tokyo
Japan, 100-6111
Phone:+81 3 5521 8500
Fax:+81 3 5521 2388
Email:DuPont.Biotech@jpn.dupont.com
Url:http://www.dupont.co.jp/bio/index.html
Description
The stacked maize line TC1507×DAS59122×MON810×MIR604×NK603 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of cry1F, pat, cry34Ab1, cry35Ab1, cry1Ab, cp4epsps, cry3Aa2 genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate herbicide and glyphosate herbicide
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
PHI8999A, PHP17662, PV-ZMBK07, PV-ZMGT32 and pZM26
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Metallothionein-like gene promoter
0.00 Kb
 
 
mCry3A
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Cry34Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
Peroxidase gene promoter
0.00 Kb
 
 
Cry35Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F gene to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

DNA insert from 59122 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from MON810 vector PV-ZMBK07
MON810 contains a truncated portion of a synthetic form of the cry1Ab gene which confers resistance against lepidoptera

DNA insert from NK603 vector PV-ZMGT32
Contributed 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate. The glyphosate-tolerant EPSPS gene was isolated from the CP4 strain of the common soil bacterium Agrobacterium tumefaciens.

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Mannose tolerance
Common use(s)
  • Food
  • Feed
Additional Information

Records referencing this document (9)
IDDescription
9record(s) found
Country's Decision or any other Communication2 records
Information Resource2 records
Organization1 record
Risk Assessment4 records