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Modified Organism
MON-8746Ø-4 x MON-89Ø34-3 x MON-ØØ6Ø3-6 - Maize Modified for drought and glyphosate tolerance and resistance to Lepidoptera
Record information and status
Record ID
104680
Status
Published
Date of creation
2013-02-07 21:44 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-07-24 18:54 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 18:54 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize Modified for drought and glyphosate tolerance and resistance to Lepidoptera
Transformation event
MON87460 x MON89034 x NK603
Unique identifier
MON-8746Ø-4 x MON-89Ø34-3 x MON-ØØ6Ø3-6
Developer(s)
Monsanto Japan Limited
Ginza Sanno Bldg. 8F  4-10-10, Ginza
Chuo-ku, Tokyo
Japan, 104-0061
Url:http://www.monsanto.co.jp/
Description
This stacked maize line contains genetic elements that were inherited via the traditional cross breeding of the parental lines MON87460, MON89034 and NK603. This line expresses the cspB, cry1A.105 and cry2Ab2 proteins. These proteins are to confer drought and glyphosate tolerance and resistance to Lepidoptera.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
MON-89Ø34-3 - YieldGard™ VT Pro™
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
MON-ØØ6Ø3-6 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-8746Ø-4 - Droughtgard™ Maize
Resistance to antibiotics - Kanamycin Tolerance to abiotic stress - Cold / Heat, Drought
Show detection method(s)
Related LMOs
MON-89Ø34-3 x MON-ØØ6Ø3-6 - Genuity® VT Double Pro™ Maize
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMAP595, PV-ZMIR245 and PV-ZMGT32
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.30 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry1A.105
3.53 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
FMV 35S promoter
0.56 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Transit peptide and first intron of Rubisco SSU
0.40 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Rice actin 1 gene promoter
0.80 Kb
 
 
Rice actin 1, intron
0.60 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
CaMV Enhanced 35S promoter
0.60 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.20 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.30 Kb
 
 
Rice actin 1 gene promoter
0.92 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cold shock protein gene
0.20 Kb
 
 
Transcript 7 gene 3' untranslated region
0.51 Kb
 
 
loxP recombination site
0.03 Kb
 
 
CaMV 35S promoter
0.29 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
loxP recombination site
0.03 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON87460 vector PV-ZMAP595
MON 87460 expresses cold shock protein B (CspB) from Bacillus subtilis and NptII from Tn5 of Escherichia coli. It was developed to provide reduced yield loss under water-limited conditions compared to conventional maize.

DNA insert from MON89034 vector PV-ZMIR245
Maize line MON89034 expresses two Bt-toxins encoded by the genes cry1A.105 and cry2Ab2 from Bacillus thuringiensis that confer resistance against certain lepidopteran pests such as fall armyworm (Spodoptera sp.), black cutworm (Agrotis ipsilon), european corn borer (Ostrinia nubilalis) and the corn earworm (Helicoverpa zea).

DNA insert from NK603 vector PV-ZMGT32
NK603 contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows the plant to survive the otherwise lethal application of glyphosate.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (10)
IDDescription
10record(s) found
Country's Decision or any other Communication4 records
Information Resource1 record
Risk Assessment5 records