Record details

Living Modified Organism identity

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LMO name

Vistive Gold™ Soybean

Transformation event

MON 87705

Unique identifier

MON-877Ø5-6

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Description

The modified fatty acid profile in MON 87705 soybean oil is achieved through the use of endogenous soybean (Glycine max L.) gene segments configured to suppress FATB and FAD2 gene expression. MON 87705 contains FATB1-A and FAD2-1A gene segments under the control of a seed promoter, limiting oil composition modification to this tissue.

The assembled gene transcript has an inverted repeat that produces double stranded RNA (dsRNA) that, via the RNA interference (RNAi) pathway, suppresses endogenous FATB and FAD2 gene expression, thereby producing the desired fatty acid phenotype

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #10453

Glycine max - Soybean, Soya bean, Soya, SOYBN

Characteristics of the transformation process

Vector

PV-GMPQ/HT4404

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

FMV 35S Enhancer

#105196

0.00 Kb

Elongation factor EF-1alpha promoter

#103903

0.00 Kb

Elongation factor EF-1alpha Leader

#103904

0.05 Kb

Elongation factor EF-1alpha Intron 1

#103905

0.62 Kb

Chloroplast transit peptide 2

#100365

0.23 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.37 Kb

rbcS-E9 gene terminator

#101877

0.64 Kb

α' subunit of β-conglycinin gene promoter

#104359

0.84 Kb

delta(12)-fatty acid dehydrogenase

#100267

0.27 Kb

Palmitoyl acyl carrier protein thioesterase 5'UTR

#104682

0.30 Kb

Palmitoyl acyl carrier protein thioesterase 5'UTR

#104682

0.30 Kb

delta(12)-fatty acid dehydrogenase

#100267

0.27 Kb

H6 gene 3'UTR

#104689

0.44 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The PV-GMPQ/HT4404 vector contains two T-DNAs, each delineated by left and right border regions.

T-DNA I contains a a codon optimised cp4 epsps expression cassette and a partial suppression cassette. The cp4 epsps expression cassette is under the regulation of FMV/Tsf1 chimeric promoter (1.039 kbp) and E9 polyadenylation sequence. The partial suppression cassette in T-DNA I contains the sense segments of the FAD2-1A intron and FATB1-A 5' UTR, including the chloroplast targeting sequence, which are under the regulation of the seed 7Sα' promoter.

T-DNA II contains a partial suppression cassette, which consists of the antisense segment of FAD2-1A intron and FATB1-A 5′ UTR that is flanked by the H6 untranslated sequence.

During plant transformation, a portion of the plants that were generated contained the two T-DNAs co-integrated at one locus in the soybean genome creating a DNA insert that contains a cp4 epsps cassette and a single FAD2-1A and FATB1-A suppression cassette.

LMO characteristics

Modified traits