Vistive Gold™ Soybean

The modified fatty acid profile in MON 87705 soybean oil is achieved through the use of endogenous soybean (Glycine max L.) gene segments configured to suppress FATB and FAD2 gene expression.  MON 87705 contains FATB1-A and FAD2-1A gene segments under the control of a seed promoter, limiting oil composition modification to this tissue. 

The assembled gene transcript has an inverted repeat that produces double stranded RNA (dsRNA) that, via the RNA interference (RNAi) pathway, suppresses endogenous FATB and  FAD2  gene expression, thereby producing the  desired fatty acid phenotype

PV-GMPQ/HT4404

The PV-GMPQ/HT4404 vector contains two T-DNAs, each delineated by left and right border regions. 

T-DNA I contains a a codon optimised cp4 epsps expression cassette and a partial suppression cassette. The  cp4 epsps expression cassette is under the regulation of FMV/Tsf1 chimeric promoter (1.039 kbp) and E9 polyadenylation sequence.  The partial suppression cassette in T-DNA I contains the sense segments of the FAD2-1A intron and FATB1-A 5' UTR, including the chloroplast targeting sequence, which are under the regulation of the seed 7Sα' promoter.

T-DNA II contains a partial suppression cassette, which consists of the antisense segment of FAD2-1A intron and FATB1-A 5′ UTR that is flanked by the H6 untranslated sequence.

During  plant transformation, a portion of the plants that were generated contained the two T-DNAs co-integrated at one locus in the soybean genome creating a DNA insert that contains a  cp4 epsps cassette and a single  FAD2-1A and FATB1-A suppression cassette.