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Modified Organism
MON-877Ø5-6 - Vistive Gold™ Soybean
Record information and status
Record ID
104683
Status
Published
Date of creation
2013-02-08 19:36 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-01-22 20:12 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 20:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Vistive Gold™ Soybean
Transformation event
MON 87705
Unique identifier
MON-877Ø5-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The modified fatty acid profile in MON 87705 soybean oil is achieved through the use of endogenous soybean (Glycine max L.) gene segments configured to suppress FATB and FAD2 gene expression.  MON 87705 contains FATB1-A and FAD2-1A gene segments under the control of a seed promoter, limiting oil composition modification to this tissue. 

The assembled gene transcript has an inverted repeat that produces double stranded RNA (dsRNA) that, via the RNA interference (RNAi) pathway, suppresses endogenous FATB and  FAD2  gene expression, thereby producing the  desired fatty acid phenotype
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Characteristics of the transformation process
Vector
PV-GMPQ/HT4404
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
0.00 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.05 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.62 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
 
α' subunit of β-conglycinin gene promoter
0.84 Kb
 
 
delta(12)-fatty acid dehydrogenase
0.27 Kb
 
 
Palmitoyl acyl carrier protein thioesterase 5'UTR
0.30 Kb
 
 
Palmitoyl acyl carrier protein thioesterase 5'UTR
0.30 Kb
 
 
delta(12)-fatty acid dehydrogenase
0.27 Kb
 
 
H6 gene 3'UTR
0.44 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The PV-GMPQ/HT4404 vector contains two T-DNAs, each delineated by left and right border regions. 

T-DNA I contains a a codon optimised cp4 epsps expression cassette and a partial suppression cassette. The  cp4 epsps expression cassette is under the regulation of FMV/Tsf1 chimeric promoter (1.039 kbp) and E9 polyadenylation sequence.  The partial suppression cassette in T-DNA I contains the sense segments of the FAD2-1A intron and FATB1-A 5' UTR, including the chloroplast targeting sequence, which are under the regulation of the seed 7Sα' promoter.

T-DNA II contains a partial suppression cassette, which consists of the antisense segment of FAD2-1A intron and FATB1-A 5′ UTR that is flanked by the H6 untranslated sequence.

During  plant transformation, a portion of the plants that were generated contained the two T-DNAs co-integrated at one locus in the soybean genome creating a DNA insert that contains a  cp4 epsps cassette and a single  FAD2-1A and FATB1-A suppression cassette.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (24)
IDDescription
24record(s) found
Country's Decision or any other Communication10 records
Modified Organism2 records
Organization2 records
Risk Assessment10 records