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Modified Organism
MON-883Ø2-9 - TruFlex Roundup Ready™ Canola
Record information and status
Record ID
104752
Status
Published
Date of creation
2013-03-13 19:38 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-01-22 20:27 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 20:27 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
TruFlex Roundup Ready™ Canola
Transformation event
MON88302
Unique identifier
MON-883Ø2-9
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
MON 88302 produces the 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein, via the incorporation of a cp4 epsps coding sequence. The CP4 EPSPS protein confers tolerance to the herbicide glyphosate, the active ingredient in the family of Roundup® agricultural herbicides.

MON 88302 utilizes a FMV/Tsf1 chimeric promoter sequence to drive CP4 EPSPS expression in different plant tissues. By virtue of CP4 EPSPS expression in pollen, MON 88302 provides tolerance to glyphosate during the sensitive reproductive stages of growth, and enables the application of glyphosate at higher rates up to first flower with no detectable impact to male fertility.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Variety: Ebony
Characteristics of the transformation process
Vector
PV-BNHT2672
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
1.04 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.05 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.62 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The EPSPS gene is a codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4.

Southern blot analysis indicated that a single copy of the T-DNA was integrated at a single locus in the genome with no integration of fragments from the backbone vector.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Detection method(s)
External link(s)

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication6 records
Modified Organism2 records
Risk Assessment8 records