Record details

Living Modified Organism identity

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LMO name

TruFlex Roundup Ready™ Canola

Transformation event

MON88302

Unique identifier

MON-883Ø2-9

Developer(s)

Record #14925

Monsanto

800 North Lindbergh Blvd.

St. Louis, MO

United States of America, 63167

Phone:	+ 1 314 694-1000
Fax:	+1 314 694-3080
Url:	Monsanto

Description

MON 88302 produces the 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) protein, via the incorporation of a cp4 epsps coding sequence. The CP4 EPSPS protein confers tolerance to the herbicide glyphosate, the active ingredient in the family of Roundup® agricultural herbicides.

MON 88302 utilizes a FMV/Tsf1 chimeric promoter sequence to drive CP4 EPSPS expression in different plant tissues. By virtue of CP4 EPSPS expression in pollen, MON 88302 provides tolerance to glyphosate during the sensitive reproductive stages of growth, and enables the application of glyphosate at higher rates up to first flower with no detectable impact to male fertility.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #12083

Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA

Point of collection or acquisition of the recipient organism

Variety: Ebony

Characteristics of the transformation process

Vector

PV-BNHT2672

Techniques used for the modification

Agrobacterium-mediated DNA transfer

Genetic elements construct

FMV 35S Enhancer

#105196

1.04 Kb

Elongation factor EF-1alpha promoter

#103903

0.00 Kb

Elongation factor EF-1alpha Leader

#103904

0.05 Kb

Elongation factor EF-1alpha Intron 1

#103905

0.62 Kb

Chloroplast transit peptide 2

#100365

0.23 Kb

5-enolpyruvylshikimate-3-phosphate synthase gene

#14979

1.37 Kb

rbcS-E9 gene terminator

#101877

0.64 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

The EPSPS gene is a codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4.

Southern blot analysis indicated that a single copy of the T-DNA was integrated at a single locus in the genome with no integration of fragments from the backbone vector.

LMO characteristics

Modified traits