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Modified Organism
MON-87427-7 - Maize modified for tissue selective glyphosate tolerance
Record information and status
Record ID
104758
Status
Published
Date of creation
2013-03-14 19:44 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-01-22 20:09 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 20:09 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for tissue selective glyphosate tolerance
Transformation event
MON87427
Unique identifier
MON-87427-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
MON87427 was modifies to express the CP4 EPSPS protein which confers tolerance to the herbicide glyphosate.

The e35S-hsp70 promoter and intron combination is used to drive the tissue selective expression of the cp4 epsps gene resulting in CP4 EPSPS protein production in vegetative and female reproductive tissue, providing tolerance to glyphosate within these tissues. This specific promoter and intron combination also results in limited or no production of CP4 EPSPS protein in two key male reproductive tissues: pollen microspores which develop into pollen grains, and tapetum cells that supply nutrients to the pollen.

Thus, in MON 87427, male reproductive tissues critical for male gametophyte development are not tolerant to glyphosate. This allows glyphosate-treated MON 87427 containing inbred lines to serve as a female parent in the production of hybrid seed.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Cultivar: LH198 × HiII
Characteristics of the transformation process
Vector
PV-ZMAP1043
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The cp4 epsps coding sequence is the codon optimized coding sequence of the aroA gene from Agrobacterium sp. strain CP4 encoding CP4 EPSPS.

Southern blot analyses indicate that a single copy of the T-DNA was inserted at a single site in the maize genome and no plasmid vector backbone sequences were detected to have been integrated. DNA sequencing analyses indicated that the expected T-DNA sequences were integrated.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (20)
IDDescription
20record(s) found
Country's Decision or any other Communication7 records
Modified Organism5 records
Risk Assessment8 records