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Gene and DNA Sequence
Record information and status
Record ID
104789
Status
Published
Date of creation
2013-03-22 20:14 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-02-24 19:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-02-24 19:24 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
eCry3.1Ab
Abbreviation
CS-eCry3_1Ab-BACTU
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
Yes
Donor organism
Donor organism
Bacillus thuringiensis - Bt, Bacillus, BACTU
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
eCry3.1Ab delta-endotoxin
Biological function
An engineered Cry gene active against certain corn rootworm (Diabrotica) species . The gene ecry3.1Ab consists of a fusion between the 5′ end (Domain I, Domain II and 15 amino acids of Domain III) of a modified Cry3A gene (mcry3A) and the 3′ end (Domain III and Variable Region 6) of a synthetic Cry1Ab gene

Upstream of the mcry3A domain, the gene ecry3.1Ab carries a 67-bp-long oligomer extension at its 5′ end, which was introduced during the engineering of the variable regions and is translated into the following 22 amino acid residues: MTSNGRQCAGIRPYDGRQQHRG. The next 459 amino acid residues are identical to a portion of mCry3A, followed by 172 residues that are identical to a portion of Cry1Ab.
Related trait(s) or use(s) in biotechnology

Records referencing this document (42)
IDDescription
42record(s) found
Country's Decision or any other Communication16 records
Modified Organism11 records
Risk Assessment15 records