| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
SYN-Ø53Ø7-1 - Agrisure® Duracade™ Maize
Record information and status
Record ID
104791
Status
Published
Date of creation
2013-03-25 13:27 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-03-19 16:22 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-03-19 16:23 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Agrisure® Duracade™ Maize
Transformation event
Event 5307
Unique identifier
SYN-Ø53Ø7-1
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Description
Event 5307 corn plants contain the transgene ecry3.1Ab encoding a novel rootworm-control protein, eCry3.1Ab, and the transgene pmi encoding the enzyme phosphomannose isomerase (PMI).

The eCry3.1Ab protein is an engineered chimera of the modified Cry3A (mCry3A) and Cry1Ab proteins, members of a class of insecticidal proteins derived from Bacillus thuringiensis(Bt). The gene pmi was obtained from Escherichia colistrain K-12 and the protein it encodes was utilized as a plant selectable marker during development of 5307 corn.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Corn line: NP2222
Characteristics of the transformation process
Vector
pSYN12274
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Cestrum Yellow Leaf Curling Virus promoter
0.35 Kb
 
 
eCry3.1Ab
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Southern blot analysis indicated that a single intact copy of the transformation cassette was inserted into the maize genome and that there was no integration of any backbone vector fragments. Nucleotide sequencing indicated that the the regulatory and functional elements of the insert were the same as those present in the pSYN12274 plasmid. Sequence analysis also revealed that some truncation occurred at the right border (RB) and left border (LB) ends of the T-DNA during the transformation process.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed

Records referencing this document (15)
IDDescription
15record(s) found
Country's Decision or any other Communication6 records
Modified Organism3 records
Organization1 record
Risk Assessment5 records