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Gene and DNA Sequence
Record information and status
Record ID
104793
Status
Published
Date of creation
2013-03-25 15:28 UTC (dina.abdelhakim@cbd.int)
Date of last update
2014-08-11 20:27 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-11 20:27 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
4-Hydroxyphenylpyruvate dioxygenase gene
Abbreviation
CS-hppdPfW336-PSEFL
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
No
Donor organism
Donor organism
Pseudomonas fluorescens - PSEFL
Donor organism point of collection
Strain: A32
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
4-hydroxyphenylpyruvate dioxygenase
Biological function
4-hydroxyphenylpyruvate dioxygenase (HPPD) catalyses the conversion of 4-hydroxyphenylpyruvate to homogentisate which is the aromatic precursor of tocopherol and plastoquinone, which are essential to the photosynthetic transport chain and antioxidative systems. This reaction involves decarboxylation, substituent migration and aromatic oxygenation in a single catalytic cycle.

To enhance the protein's tolerance against HPPD inhibitors a point mutation was introduced into the coding sequence such that a replacement of the amino acid glycine with a tryptophane at position 336 was introduced, as described by  Boudec et al. (2001)
Related trait(s) or use(s) in biotechnology
  • Resistance to herbicides
Additional Information
Additional Information
In plants inhibition of HPPD affects the formation of homogentisic acid, which is a key precursor for the biosynthesis of both tocopherols (vitamin E) and plastoquinone, a critical co-factor in the formation of carotenoids, which protect chlorophyll in plants from being destroyed by sunlight. If this happens,the plants turn white without deformation due to complete loss of chlorophyll, and the plant dies.

Records referencing this document (20)
IDDescription
20record(s) found
Country's Decision or any other Communication9 records
Modified Organism2 records
Risk Assessment9 records