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Modified Organism
MST-FGØ72-2 - Soy modified for tolerance to glyphosate and HPPD inhibitors
Record information and status
Record ID
104794
Status
Published
Date of creation
2013-03-25 15:54 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-03-25 15:54 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Soy modified for tolerance to glyphosate and HPPD inhibitors
Transformation event
FG72
Unique identifier
MST-FGØ72-2
Developer(s)
Bayer CropScience LP
2 T.W. Alexander Dr.
Research Triangle Park, NC
United States of America, 27709
Phone:+919-549-2599
Fax:+919-549-3929
Description
Soy FG72 was modified with the insertion of expression cassettes that code for a mutated form of epsps that confers tolerance to the glyphosate herbicide and a modified form of the hppd gene that is tolerant to HPPD inhibitors such as isoxaflutole (IFT).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Cultivar: Jack
Characteristics of the transformation process
Vector
pSF10
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Histone H4 gene Promoter
1.01 Kb
 
 
Histone H3 Gene II intron 1
0.48 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
Double Mutant 5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Histone H4 gene 3' UTR
0.69 Kb
 
 
Histone H4 gene Promoter
1.29 Kb
 
 
TEV 5' Untranslated Region
0.14 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
4-Hydroxyphenylpyruvate dioxygenase gene
1.08 Kb
 
 
Nopaline Synthase Gene Terminator
0.29 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The 2mepsps expression cassette was inserted into the vector in an antisense orientation relative to the hppdPfW336 expression cassette.

The version of the histone H4 gene promoter used to drive the hppdPfW336 gene contains an internal portion of the promoter sequence (referred to as "B") was duplicated to increase the promoter activity in plant cells.

Southern blot ananlysis indicated that two partial 3'histonAt sequences in a head to head orientation at the 5' end of the insert followed by 2 complete copies of the linear fragment excised from plasmid pSF10 arranged in a head to tail orientation were integrated into the host genome. Upon integration of the FG72 insert into the soybean genome, a genomic region translocated to a new position, which is joined at the 3' junction by 158 bases of Ph4a748 promoter sequences.

Analysis also indicated that there was no integration of fragments of the vector backbone.
LMO characteristics
Modified traits
  • HPPD Herbicides
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (15)
IDDescription
15record(s) found
Country's Decision or any other Communication6 records
Modified Organism1 record
Organization2 records
Risk Assessment6 records