Maize modified for insect resistance and herbicide tolerance

The stacked maize line DP-Ø9814Ø-6, DAS-Ø15Ø7-1 and DAS-59122-7 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of the cry1F, cry34Ab1, cry35Ab1, gat4621, phosphinothricin acetyltransferase, and acetohydroxyacid synthase genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate, Sulfonylurea and glyphosate herbicide.

PHP17662, PHP24279 and PHI8999A

DNA insert from DAS-59122-7 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from DP-Ø9814Ø-6 vector PHP24279
Expresses the GAT4621 and ZM-HRA proteins. The GAT4621 protein is a glyphosate acetyltransferase (GAT), confers tolerance to the herbicide glyphosate. The acetohydroxyacid synthase (AKA acetolactate synthase) gene confers tolerance to ALS-inhibiting herbicides, such as chlorimuron and thifensulfuron.

DNA insert from DAS-Ø15Ø7-1 vector PHI8999A
Expresses the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

For additional information on this LMO, please refer to the records of the parental LMOs.