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Modified Organism
DP-Ø9814Ø-6 x DAS-Ø15Ø7-1 x DAS-59122-7 - Maize modified for insect resistance and herbicide tolerance
Record information and status
Record ID
104815
Status
Published
Date of creation
2013-03-28 15:50 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-07-24 19:11 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 19:11 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for insect resistance and herbicide tolerance
Transformation event
98140 x TC1507 x 59122
Unique identifier
DP-Ø9814Ø-6 x DAS-Ø15Ø7-1 x DAS-59122-7
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The stacked maize line DP-Ø9814Ø-6, DAS-Ø15Ø7-1 and DAS-59122-7 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of the cry1F, cry34Ab1, cry35Ab1, gat4621, phosphinothricin acetyltransferase, and acetohydroxyacid synthase genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate, Sulfonylurea and glyphosate herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
DP-Ø9814Ø-6 - Optimum™ GAT™ maize
Resistance to herbicides - Glyphosate, Sulfonylurea
Show detection method(s)
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
PHP17662, PHP24279 and PHI8999A
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
Proteinase inhibitor II gene terminator
0.31 Kb
 
 
Acetohydroxyacid synthase gene
1.92 Kb
 
 
Acetohydroxyacid synthase gene promoter
0.66 Kb
 
 
Ubiquitin gene promoter
0.90 Kb
 
 
Ubiquitin 5' Untranslated Region
0.08 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Glyphosate-N-Acteyltransferase gene
0.44 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Cry34Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
Peroxidase gene promoter
0.00 Kb
 
 
Cry35Ab1
0.00 Kb
 
 
Proteinase inhibitor II gene terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from DAS-59122-7 vector PHP17662
The cry34Ab1 and cry35Ab1 genes, isolated from the common soil bacterium Bacillus thuringiensis (Bt) strain PS149B1, produce the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1. The pat gene was isolated from the soil bacterium Streptomyces viridochromogenes and confers tolerance to herbicides containing glufosinate ammonium.

DNA insert from DP-Ø9814Ø-6 vector PHP24279
Expresses the GAT4621 and ZM-HRA proteins. The GAT4621 protein is a glyphosate acetyltransferase (GAT), confers tolerance to the herbicide glyphosate. The acetohydroxyacid synthase (AKA acetolactate synthase) gene confers tolerance to ALS-inhibiting herbicides, such as chlorimuron and thifensulfuron.

DNA insert from DAS-Ø15Ø7-1 vector PHI8999A
Expresses the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Information Resource1 record
Risk Assessment1 record