Papaya modified for delayed ripening | BCH-LMO-SCBD-104889 | Living Modified Organism | Biosafety Clearing-House

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Living Modified Organism (LMO)
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Decisions on the LMO Risk Assessments  
last updated: 03 Jun 2013
Living Modified Organism identity
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Papaya modified for delayed ripening
EN
Transgenic Eksotika papaya
No
The transgenic Eksotika papaya is developed by inserting an antisense portion of the aminocyclopropanecarboxylate oxidase (ACO2/ ACC oxidase) coding sequence resulting in the antisense suppression of endogenous ACC oxidase enzyme. This results in delayed fruit ripening by inhibiting the production of ethylene gas.

The transgenic Eksotika papaya was also transformed with the nptII gene. The NPTII enzyme confers resistance to kanamycin and is used to select transformed cells.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
Variety: Eksotika
EN
Characteristics of the modification process
pASACO2E1 (derived from pGA643)
EN
  • Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
  • BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)
    Promoter
  • BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)
    Protein coding sequence | Resistance to antibiotics (Kanamycin)
  • BCH-GENE-SCBD-104887-2 Aminocyclopropanecarboxylate oxidase gene | Carica papaya (Papaya, Pawpaw, Papaw, CARPA)
    Protein coding sequence | Changes in physiology and/or production (Ripening)
  • BCH-GENE-SCBD-103067-9 Transcript 7 gene 3' untranslated region | Agrobacterium tumefaciens (Agrobacterium)
    Terminator
  • BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)
    Promoter
Aminocyclopropanecarboxylate oxidase (ACC oxidase) coding sequence in inserted in an antisense orientation thus resulting in the antisense suppression of endogenous ACC oxidase production.
EN
LMO characteristics
EN
  • Food
  • Research
Detection method(s)
EN
Additional Information
EN
Records referencing this document Show in search
Record type Field Record(s)
Risk Assessment generated by a regulatory process Living modified organism(s) 1
Country's Decision or any other Communication Living modified organism(s) 1