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Modified Organism
Papaya modified for delayed ripening
Record information and status
Record ID
104889
Status
Published
Date of creation
2013-06-03 07:56 UTC (anita@nre.gov.my)
Date of publication
2013-06-03 16:38 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Papaya modified for delayed ripening
Transformation event
Transgenic Eksotika papaya
Developer(s)
Ms Rogayah Sekeli
Research Officer
Biotechnology Research Centre
Malaysian Agricultural Research and Development Institute (MARDI)
Biotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400
Serdang, Selangor
Malaysia, 43400
Phone:+60389437645
Fax:+60389437630
Email:lynn@mardi.gov.my
Url:Official Portal Malaysian Agricultural Research and Development Institute
Description
The transgenic Eksotika papaya is developed by inserting an antisense portion of the aminocyclopropanecarboxylate oxidase (ACO2/ ACC oxidase) coding sequence resulting in the antisense suppression of endogenous ACC oxidase enzyme. This results in delayed fruit ripening by inhibiting the production of ethylene gas.

The transgenic Eksotika papaya was also transformed with the nptII gene. The NPTII enzyme confers resistance to kanamycin and is used to select transformed cells.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Carica papaya - Papaya, Pawpaw, Papaw, CARPA
Point of collection or acquisition of the recipient organism
Variety: Eksotika
Characteristics of the transformation process
Vector
pASACO2E1 (derived from pGA643)
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Aminocyclopropanecarboxylate oxidase gene
0.00 Kb
 
 
Transcript 7 gene 3' untranslated region
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Aminocyclopropanecarboxylate oxidase (ACC oxidase) coding sequence in inserted in an antisense orientation thus resulting in the antisense suppression of endogenous ACC oxidase production.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record