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Modified Organism
DP-ØØ4114-3 - Insect-Resistant and Herbicide-Tolerant Maize
Record information and status
Record ID
104976
Status
Published
Date of creation
2013-07-18 18:50 UTC (dina.abdelhakim@cbd.int)
Date of last update
2013-09-23 20:18 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-23 20:19 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect-Resistant and Herbicide-Tolerant Maize
Transformation event
4114
Unique identifier
DP-ØØ4114-3
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
A stacked insect-resistant and herbicide-tolerant maize. Insect resistance is conferred by the cry34Ab1, cry35Ab1 and cryIF genes from the common soil bacterium Bacillus thuringiensis, and glufosinate-ammonium herbicide tolerance through the pat gene from Streptomyces viridochromogenes.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Maize inbred line: PHWWE
Related LMOs
DAS-59122-7 x DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6 - Herculex XTRA™ Roundup Ready™ 2 maize
Resistance to diseases and pests - Insects - Coleoptera (beetles), Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate, Glyphosate
Show detection method(s)
DP-Ø32316-8 - Insect Resistant, Herbicide Tolerant Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles), Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
PHP27118
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.71 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.31 Kb
 
 
Peroxidase gene promoter
1.29 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.31 Kb
 
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.19 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The [poly]ubiquitin gene promoter element is made up of  the promoter region (900bp) and the 5' untranslated region (83bp) of the [poly]ubiquitin gene

Southern blot analyses indicated that a single, intact transformation cassette was inserted into the genome of 4114 maize with no integration of the backbone of plasmid PHP27118.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (10)
IDDescription
10record(s) found
Country's Decision or any other Communication4 records
Modified Organism2 records
Risk Assessment4 records