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Modified Organism
Female-specific Dominant Lethal Olive Fly
Record information and status
Record ID
105039
Status
Published
Date of creation
2013-09-17 19:01 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-17 19:01 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Female-specific Dominant Lethal Olive Fly
Transformation event
OX3097D-Bol
Developer(s)
Oxitec Limited
71 Milton Park
OX14 4RX
Oxford, England
Phone:+44 (0) 1235 832393
Fax:+44 (0) 1235 861138
Email:info@oxitec.com
Url:http://www.oxitec.com
Description
A transgenic strain of the olive fly, designated as OX3097D-Bol, was developed to exhibit dominant, female-specific lethality when reared in the absence of a transgene repressor as well as a dominant fluorescent marking to identify the transgenic insects.

Mating of released males with wild females will result in the survival of male OX3097D‐Bol olive flies however all the females will fail to develop further than the late larval stages.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Bactrocera (Dacus) oleae - Olive fruit fly, Olive fly
Characteristics of the transformation process
Vector
OX3097
Techniques used for the modification
  • Microinjection
Genetic elements construct
 
Transcription termination factor
0.00 Kb
 
 
DsRed2 Fluorescent Protein
0.00 Kb
 
 
Immediate-early-1 gene promoter
0.00 Kb
 
 
hr5 Transcriptional Enhancer
0.00 Kb
 
 
tet Operator Sequences
0.00 Kb
 
 
HSP70 minimal promoter
0.00 Kb
 
 
cctra intron
0.00 Kb
 
 
Tetracycline-controlled transactivator
0.00 Kb
 
 
fs(1)K10 3' UTR
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Additional Information
Information on the Release of Insects carrying a Dominant Lethal (RIDL) technology

Release of Insects carrying a Dominant Lethal (RIDL) is a method using recombinant DNA technology to create genetically modified insects for biological control. The dominant lethal gene kills the insects but it can be repressed by an external additive, which allows the insects to be reared in manufacturing facilities. This external additive is commonly administered orally, and so can be an additive to the insect food. The insects can also be given genetic markers, such as fluorescence, that make monitoring the progress of eradication easier.

There are potentially several types of RIDL, but the more advanced forms have a female-specific dominant lethal gene. This avoids the need for a separate sex separation step, as the repressor can be withdrawn from the final stage of rearing, leaving only males.

These males are then released in large numbers into the affected region. The released males are not sterile, but any female offspring their mates produce will have the dominant lethal gene expressed, and so will die. The number of females in the wild population will therefore decline, causing the overall population to decline.

Using RIDL means that the males will not have to be sterilized by radiation before release (as done with the "Sterile Insect Technique" (SIT) using radiation), making the males healthier when they need to compete with the wild males for mates.