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Modified Organism
DP-Ø61Ø61-7 - Glyphosate tolerant canola
Record information and status
Record ID
105040
Status
Published
Date of creation
2013-09-18 15:43 UTC (dina.abdelhakim@cbd.int)
Date of last update
2021-02-18 17:21 UTC (austein.mcloughlin@cbd.int)
Date of publication
2021-02-18 17:22 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Glyphosate tolerant canola
Transformation event
61061
Unique identifier
DP-Ø61Ø61-7
Developer(s)
Pioneer Hi-Bred International Inc.
7100 NW 62nd Avenue
PO Box 1000
Johnston, Iowa
United States of America, 50131
Phone:+1 515 535-3200
Url:Pioneer HiBred International Homepage
Description
The canola (Brassica rapa) was modified for tolerance to the glyphosate herbicide through the insertion of the Bacillus licheniformis glyphosate-N-acteyltransferase gene, which inactivates glyphosate through acetylation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica rapa - Canola plant
Related LMOs
DP-Ø73496-4 - Optimum® Gly Canola
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PHP28181
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Polyubiquitin10 gene promoter
1.31 Kb
 
 
Glyphosate-N-Acteyltransferase gene
0.44 Kb
 
 
Proteinase inhibitor II gene terminator
0.31 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The canola contains one gene cassette for Bacillus licheniformis glyphosate-N-ccteyltransferase (gat). The coding sequence was under control of an Arabidopsis thaliana ubiquitin-10 promoter and a Solanum tuberonsum proteinase inhibitor II 3' untranslated region.

The gat coding sequence expresses the GAT4621 version of the protein, which was functionally improved by a gene shuffling process to optimize the kinetics of glyphosate acetyltransferase.

Southern blot analysis indicated that a single, intact PHP28181 DNA fragment was inserted into the genome with no plasmid backbone DNA.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Detection method(s)
Additional information
See patent in additional information section below

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment2 records