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Modified Organism
DAS-81419-2 - Herbicide tolerant, Insect resistant Soybean
Record information and status
Record ID
105046
Status
Published
Date of creation
2013-09-23 15:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-09-23 15:24 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant, Insect resistant Soybean
Transformation event
DAS-81419-2
Unique identifier
DAS-81419-2
Developer(s)
Description
Soy line DAS-81419-2 was transformed with the insertion of Cry1F and Cry1Ac to confer resistance to lepidopteran insects as well as the insertion of the pat gene which results in the synthesis of phosphinothricin N-acetyltransferase thus coffering tolerance to glufosinate herbicides.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Cultivar: Maverick
Characteristics of the transformation process
Vector
pDAB9582
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Polyubiquitin10 gene promoter
1.32 Kb
 
 
Cry1F
3.45 Kb
 
 
ORF23 3' Untranslated region
0.46 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Cry1Ac
3.47 Kb
 
 
ORF23 3' Untranslated region
0.48 Kb
 
 
CsVMV promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
ORF1 3' Untranslated region
0.70 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The Cry1Ac and Cry1F sequences are synthetic versions of the native genes.

The cry1Fv3 gene is comprised of three parts; at the 5′ end, a toxin core that was optimized from the native cry1Fa2 gene originally isolated from Bacillus thuringiensis subsp. aizawai strain PS811; in the middle, a very small portion of cry1Ca3 which was originally isolated from B. thuringiensis subsp. aizawai strain PS811; and at the 3′ end, a tail that was optimized from the native cry1Ab1 tail originally isolated from B. thuringiensis subsp. Berliner 1715. The cry1Fv3 gene encodes the Cry1F protein that is comprised of 1148 amino acids and has a molecular weight of ~130.2 kDa. The amino acid sequence of the Cry1F protein is identical to that expressed in cotton event DAS-24236-5.

The cry1Ac(synpro) gene is comprised of three parts; at the 5′ end, a toxin core that was optimized from the native cry1Ac1 gene originally isolated from B. thuringiensis subsp. kurstakistrain HD73; in the middle, a very small portion of cry1Ca3 which was originally isolated from B. thuringiensis subsp. aizawai strain PS811; and at the 3′ end, a tail that was optimized from the native cry1Ab1 tail originally isolated from B. thuringiensis subsp. Berliner 1715. Thecry1Ac(synpro) gene encodes the Cry1Ac protein that is comprised of 1156 amino acids and has a molecular weight of ~130.7 kDa. The cry1Ac(synpro) gene sequence and the corresponding Cry1Ac amino acid sequence are identical to that expressed in cotton event DAS-21023-5

Molecular characterization by Southern blot analyses of DAS-81419-2 soybean confirmed that a single, intact DNA insert containing the cry1Ac, cry1F, and pat gene expression cassettes was integrated into the soybean genome and the intact DNA insert was stably inherited in the five breeding generations tested. Southern blot analyses confirmed the absence of the plasmid backbone DNA in DAS-81419-2 soybean.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records