The Cry1Ac and Cry1F sequences are synthetic versions of the native
genes.
The cry1Fv3 gene is comprised of three parts; at the 5′ end, a
toxin core that was optimized from the native cry1Fa2 gene
originally isolated from Bacillus thuringiensis subsp. aizawai
strain PS811; in the middle, a very small portion of cry1Ca3 which
was originally isolated from B. thuringiensis subsp. aizawai strain
PS811; and at the 3′ end, a tail that was optimized from the native
cry1Ab1 tail originally isolated from B. thuringiensis subsp.
Berliner 1715. The cry1Fv3 gene encodes the Cry1F protein that is
comprised of 1148 amino acids and has a molecular weight of ~130.2
kDa. The amino acid sequence of the Cry1F protein is identical to
that expressed in cotton event DAS-24236-5.
The cry1Ac(synpro) gene is comprised of three parts; at the 5′ end,
a toxin core that was optimized from the native cry1Ac1 gene
originally isolated from B. thuringiensis subsp. kurstakistrain
HD73; in the middle, a very small portion of cry1Ca3 which was
originally isolated from B. thuringiensis subsp. aizawai strain
PS811; and at the 3′ end, a tail that was optimized from the native
cry1Ab1 tail originally isolated from B. thuringiensis subsp.
Berliner 1715. Thecry1Ac(synpro) gene encodes the Cry1Ac protein
that is comprised of 1156 amino acids and has a molecular weight of
~130.7 kDa. The cry1Ac(synpro) gene sequence and the corresponding
Cry1Ac amino acid sequence are identical to that expressed in
cotton event DAS-21023-5
Molecular characterization by Southern blot analyses of DAS-81419-2
soybean confirmed that a single, intact DNA insert containing the
cry1Ac, cry1F, and pat gene expression cassettes was integrated
into the soybean genome and the intact DNA insert was stably
inherited in the five breeding generations tested. Southern blot
analyses confirmed the absence of the plasmid backbone DNA in
DAS-81419-2 soybean.
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