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Modified Organism
DAS-59132-8 - Insect resistant Herbicide tolerant Maize
Record information and status
Record ID
105177
Status
Published
Date of creation
2014-01-15 17:19 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-15 17:19 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant Herbicide tolerant Maize
Transformation event
Event 32
Unique identifier
DAS-59132-8
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The transgenic maize line DAS-59132-7 was genetically modified to resist Coleopteran insects (e.g. western corn rootworm, northern corn rootworm and Mexican corn rootworm) and to tolerate herbicides containing glufosinate ammonium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Maize line: Hi-II
Related LMOs
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
PHP17662
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis strain PS149B1 and the coding sequence of both genes has been adapted to the codon usage in maize as to achieve expression in the maize plant.

Southern blot analysis indicated that a single insert of the transformation cassette was introduced into the modified maize. A 450bp truncation of the 5' end was detected, which resulted in a 200bp truncation of the Ubiquitin gene promoter. The absence of the spectinomycin and tetracycline resistance genes from the vector backbone was shown by hybridizing the Southern blots with spc and tet probes.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Detection method(s)

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism1 record
Organization1 record