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Modified Organism
MON-ØØ179-5 - Reduced Lignin Alfalfa
Record information and status
Record ID
105598
Status
Published
Date of creation
2014-06-10 17:04 UTC (dina.abdelhakim@cbd.int)
Date of last update
2015-10-15 16:28 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-15 16:28 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Reduced Lignin Alfalfa
Transformation event
KK179
Unique identifier
MON-ØØ179-5
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Alfalfa KK179 was developed to have reduced levels of guaiacyl lignin (G), a major subunit component of total lignin, compared to conventional alfalfa at the same stage of growth.

This reduction in G lignin leads to reduced accumulation of total lignin in alfalfa through the suppression of caffeoyl CoA 3-O-methyltransferase (CCOMT), a key enzyme in the lignin biosynthetic pathway. 
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Medicago sativa - Alfalfa, Lucerne, MEDSV
Characteristics of the transformation process
Vector
PV-MSPQ12633
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Pal2 gene Promoter
0.00 Kb
 
 
Caffeoyl CoA 3-O-methyltransferase gene
0.52 Kb
 
 
Caffeoyl CoA 3-O-methyltransferase gene
0.30 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The transformation cassette in KK179 consists of inverted repeat DNA segments of the CCOMT gene derived from alfalfa. The inverted repeat sequence produces double-stranded RNA (dsRNA) which suppresses endogenous CCOMT gene expression via the RNA interference (RNAi) pathway. Suppression of the CCOMT gene expression leads to lower CCOMT protein expression resulting in reduced synthesis of G lignin subunit.

The PV-MSPQ12633  vector contained two transformation cassettes. the first consisting of the CCOMT inverted repeat sequence and the other containing the nptII coding sequence.

Southern blot analysis showed that only a single copy of the CCOMT transformation cassette was transformed into the alfalfa genome. No plasmid vector backbone sequences are present in KK179 and no DNA sequences from the nptII transformation cassette were detected.
LMO characteristics
Modified traits
Common use(s)
  • Feed
Additional Information

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Modified Organism1 record
Risk Assessment3 records