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Modified Organism
SYN-ØØØH2-5 - Soy modified for tolerance to Mesotrione and Glufosinate
Record information and status
Record ID
105619
Status
Published
Date of creation
2014-06-12 21:12 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-06-12 21:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Soy modified for tolerance to Mesotrione and Glufosinate
Transformation event
SYHT0H2
Unique identifier
SYN-ØØØH2-5
Developer(s)
Syngenta Seeds GmbH
Syngenta Seeds GmbH
Zum Knipkenbach 20
Bad Salzuflen
Germany, 32107
Phone:+49 52 22 5308-0
Fax:+49 52 22 5308-12
Email:info.seeds@syngenta.com
Url:Syngenta Seeds Germany
Bayer CropScience LP
2 T.W. Alexander Dr.
Research Triangle Park, NC
United States of America, 27709
Phone:+919-549-2599
Fax:+919-549-3929
Description
Soybean SYHT0H2, has been developed to be tolerant to two herbicides with different modes of action, namely glufosinate-ammonium and mesotrione.

Tolerance to glufosinate is achieved through expression of the enzyme phosphinothricin acetyltransferase (PAT). Tolerance to mesotrione is achieved through expression of the p-hydroxyphenylpyruvate dioxygenase (AvHPPD-03) protein .
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Cultivar: Jack
Characteristics of the transformation process
Vector
pSYN15954
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
0.19 Kb
 
 
CaMV 35S Enhancer
0.29 Kb
 
 
Synthetic Minimal Plant promoter
0.04 Kb
 
 
Omega 5' untranslated leader
0.07 Kb
 
 
avhppd-03 gene
1.32 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.52 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Cestrum Yellow Leaf Curling Virus promoter
0.65 Kb
 
 
Omega 5' untranslated leader
0.07 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The enzyme phosphinothricin acetyltransferase in the two different expression cassettes are encoded by two similar pat genes pat-03-01 which is regulated by the 35s promoter and pat-03-02 which is regulated by the CMP promoter. Both coding sequences encode identical PAT proteins.

The avhppd-03 was codon optimised for enhanced expression.

Southern blot and sequencing analysis indicated that SYHT0H2 contains, at a single locus, a single copy of avhppd-03, four copies of the pat gene, one copy of the avhppd-03 promoter complex, two copies of the 35s promoter, two copies of the CMP promoter, two copies of the TMV enhancer and five copies of the nos terminator sequences
LMO characteristics
Modified traits
  • Resistance to Mesotrione
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (8)
IDDescription
8record(s) found
Country's Decision or any other Communication4 records
Risk Assessment4 records