Record details

Living Modified Organism identity

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LMO name

Agrisure® Duracade™ 5222

Transformation event

5307 x MIR604 x Bt11 x TC1507 x GA21 x MIR162

Unique identifier

SYN-Ø53Ø7-1 x SYN-IR6Ø4-5 x SYN-BTØ11-1 x DAS-Ø15Ø7-1 x MON-ØØØ21-9 x SYN-IR162-4

Developer(s)

Record #101875

Syngenta Seeds GmbH

Syngenta Seeds GmbH
Zum Knipkenbach 20

Bad Salzuflen

Germany, 32107

Phone:	+49 52 22 5308-0
Fax:	+49 52 22 5308-12
Email:	info.seeds@syngenta.com
Url:	Syngenta Seeds Germany

Description

A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of 5307 x MIR604 x Bt11 x TC1507 x GA21. Insect resistance is conferred by the cry1A(b), cryIF, PAT, mcry3A, EPSPS, pmi, vip3Aa20 and eCry3.1Ab genes from the common soil bacterium Bacillus thuringiensis, and glufosinate herbicide tolerance through the pat gene from Streptomyces viridochromogenes.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #100885

SYN-IR162-4 - Agrisure™ Viptera maize

Mannose tolerance Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes

Show detection method(s)

Record #104791

SYN-Ø53Ø7-1 - Agrisure® Duracade™ Maize

Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera), Northern corn rootworm (Diabrotica barberi) Selectable marker genes and reporter genes

Record #14794

MON-ØØØ21-9 - Roundup Ready™ maize

Resistance to herbicides - Glyphosate

Show detection method(s)

Record #15105

SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize

Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes

Show detection method(s)

Record #14797

SYN-BTØ11-1 - YieldGard™ maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Record #14841

DAS-Ø15Ø7-1 - Herculex™ I maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

Show detection method(s)

Characteristics of the transformation process

Vector

pZO1502, pDPG434, pZM26, pSYN12274, pNOV1300 and PHI8999A

Techniques used for the modification

Cross breeding

Genetic elements construct

Ubiquitin gene promoter

#100362

1.99 Kb

Vegetative insecticidal protein 3Aa20

#100887

2.37 Kb

Phosphoenolpyruvate carboxylase, intron 9

#101406

0.11 Kb

CaMV 35S terminator

#100290

0.07 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Phosphomannose Isomerase gene

#15003

1.18 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Ubiquitin gene promoter

#100362

1.98 Kb

Cry1F

#14987

1.82 Kb

ORF25 PolyA Terminator sequence

#100363

0.72 Kb

CaMV 35S promoter

#100287

0.55 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

CaMV 35S terminator

#100290

0.20 Kb

CaMV 35S promoter

#100287

0.51 Kb

Alcohol Dehydrogenase 1, intron 6

#103625

0.47 Kb

Cry1Ab

#14985

1.85 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

CaMV 35S promoter

#100287

0.42 Kb

Alcohol Dehydrogenase 1, intron 2

#103867

0.18 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Rice actin 1 gene promoter

#100364

1.37 Kb

Rice actin 1, intron

#100355

0.00 Kb

Optimized Transit Peptide

#101419

0.37 Kb

5-enolpyruvylshikimate-3-phosphate synthase

#46333

1.34 Kb

Nopaline Synthase Gene Terminator

#100269

0.24 Kb

Metallothionein-like gene promoter

#103881

0.00 Kb

mCry3A

#43634

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Ubiquitin gene promoter

#100362

0.00 Kb

Ubiquitin Intron 1

#103627

0.00 Kb

Phosphomannose Isomerase gene

#15003

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Cestrum Yellow Leaf Curling Virus promoter

#104788

0.35 Kb

eCry3.1Ab

#104789

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Ubiquitin Intron 1

#103627

0.00 Kb

Phosphomannose Isomerase gene

#15003

1.18 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from Bt 11 vector pZO1502
Contributed the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from GA21 vector pDPG434
The 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene from maize was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate. The resulting gene was used to develop GA21

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F geneto confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

DNA insert from 5307 vector pSYN12274
Event 5307 corn plants contain the transgene ecry3.1Ab encoding a novel rootworm-control protein, eCry3.1Ab, and the transgene pmi encoding the enzyme phosphomannose isomerase (PMI).

DNA insert from MIR162 vector pNOV1300
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Coleoptera (beetles)
  - Lepidoptera (butterflies and moths)
Resistance to herbicides
- Glufosinate
- Glyphosate

Common use(s)

Food
Feed

Detection method(s)

External link(s)

Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR.pdf

SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

Additional Information

Other relevant website address or attached documents

SYN-Ø53Ø7-1 x SYN-IR6Ø4-5 x SYN-BTØ11-1 x DAS-Ø15Ø7-1 x MON-ØØØ21-9 x SYN-IR162-4 - ISAAA