Agrisure® Duracade™ 5222

A stacked insect-resistant and herbicide-tolerant maize derived from conventional cross-breeding of 5307 x MIR604 x Bt11 x TC1507 x GA21.  Insect resistance is conferred by the cry1A(b), cryIF, PAT, mcry3A, EPSPS, pmi, vip3Aa20 and eCry3.1Ab genes from the common soil bacterium Bacillus thuringiensis, and glufosinate herbicide tolerance through the pat gene from Streptomyces viridochromogenes.

pZO1502, pDPG434, pZM26,  pSYN12274,  pNOV1300 and PHI8999A

DNA insert from Bt 11 vector pZO1502
Contributed the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene  to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from GA21 vector pDPG434
The 5-enolpyruvyl shikimate-3-phosphate synthase (epsps) gene from maize was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate. The resulting gene was used to develop GA21

DNA insert from MIR604 vector pZM26
Cry3A gene was modified for enhanced expression in maize and such that the amino acid sequence of the synthetic version of Cry3A is the same as the native protein, except for the modified serine-protease recognition site. The pmi gene encodes the enzyme phosphomannose isomerase (PMI) that allows the plants to utilise mannose as a carbon source and is used as a selectable marker.

DNA insert from TC1507 vector PHI8999A
TC1507 modified with the insertion of the Cry1F geneto confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.

DNA insert from 5307 vector pSYN12274
Event 5307 corn plants contain the transgene ecry3.1Ab encoding a novel rootworm-control protein, eCry3.1Ab, and the transgene pmi encoding the enzyme phosphomannose isomerase (PMI).

DNA insert from MIR162 vector pNOV1300
MIR162 maize is transformed with vip3Aa20 gene which encodes the Vip3Aa20 protein that confers resistance against lepidopteran insect pests. Event MIR162 maize also contains the manA gene from Escherichia coli, which encodes the selectable marker, phosphomannose isomerase (PMI).

For additional information on this LMO, please refer to the records of the parental LMOs.