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Modified Organism
DAS-21Ø23-5 x DAS-24236-5 x MON-88913-8 x SYN-IR1Ø2-7 - Widestrike™ x Roundup Ready Flex™ x VIPCOT™ Cotton
Record information and status
Record ID
105698
Status
Published
Date of creation
2014-07-25 18:21 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-07-25 18:21 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Widestrike™ x Roundup Ready Flex™ x VIPCOT™ Cotton
Transformation event
3006-210-23 x 281-24-236 x MON88913 x COT102
Unique identifier
DAS-21Ø23-5 x DAS-24236-5 x MON-88913-8 x SYN-IR1Ø2-7
Developer(s)
Dow AgroSciences GmbH
Dow AgroSciences GmbH
Truderinger Straße 15
München, Bayern
Germany, 81677
Phone:+ 49 89 - 4 55 33 - 0
Fax:+ 49 89 - 4 55 33 - 111
Email:DowAgroSciencesD@dow.com
Url:http://www.dowagro.com/de
Description
The stacked cotton line 3006-210-23 x 281-24-236 x MON88913 x COT102 was produced by cross breeding between each of the parental organisms to resist the lepidopteran pests such as the cotton bollworm (Helicoverpa zea), amongst others, as well as tolerance against the herbicide glyphosate and glufosate via the introduction of the Cry1Ac, Cry1F, Vip3A, PAT and EPSPS genes respectively.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
DAS-24236-5 x DAS-21Ø23-5 - WideStrike™ cotton
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
SYN-IR1Ø2-7 - VIPCOT™ Cotton
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHGT35, pCOT-1, pMYC3006 and pAGM281
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
ORF25 PolyA Terminator sequence
0.00 Kb
 
 
Cry1F
0.00 Kb
 
 
4ocs∆Mas2’ promoter
0.00 Kb
 
 
4ocs∆Mas2’ promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
ORF25 PolyA Terminator sequence
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
FMV 35S Enhancer
0.00 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.00 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
CaMV 35S Enhancer
0.00 Kb
 
 
Actin 8 Promoter
0.00 Kb
 
 
Actin 8 Leader sequence
0.00 Kb
 
 
Actin 8 Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
Actin 2 Gene Promoter
0.00 Kb
 
 
Vegetative insecticidal protein 3A
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene 3 promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from COT102 vector pCOT-1:
Cotton tolerant to lepidopteran pests through introduction of the vip3A(a) gene which codes for an insecticidal protein that targets lepidopteran insect species. The aph4 gene, coding for hygromycin-B phosphotransferase (APH4) was used as a selectable marker.

DNA insert from DAS-21Ø23-5, vector pMYC3006: 
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1Ac gene from the common soil bacterium Bacillus thuringiensis (Bt) var. kurstaki strain HD73. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from DAS-24236-5, vector pAGM281:
Lepidopteran-resistant cotton, with insect resistance conferred by the cry1F gene from the common soil bacterium Bacillus thuringiensis (Bt) var. aizawai. Also contains a coding sequence or the Phosphinothricin N-acetyltransferase gene which confers tolerance to glufosinate.

DNA insert from 88913 vector PV-GHGT35:
This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Fiber / Textile
Additional Information
Other relevant website address or attached documents

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication3 records
Risk Assessment3 records