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Modified Organism
Hybrid aspen lines with modified stomatal regulation
Record information and status
Record ID
105734
Status
Published
Date of creation
2014-08-01 10:53 UTC (gtlk.stm@stm.fi)
Date of last update
2014-08-06 16:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-06 16:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Hybrid aspen lines with modified stomatal regulation
Transformation event
Multiple transformation events
Developer(s)
Division of Plant Biology and Institute of Biotechnology
Department of Biosciences
University of Helsinki
Helsinki
Finland
Phone:+358 2941 57773
Email:jorma.vahala@helsinki.fi
Description
The  hybrid aspens were genetically modified to understand the function of stomatal regulation in hybrid aspen under natural growth conditions.

In these lines the function of stomata has been modified by silencing Slow Anion Channel-Associated 1 (SLAC1) and SLAC1 homolog 3 (SLAH3) orthologs of hybrid aspen under a guard-cell-specific promoter.

The A. thaliana SLAC1 gene is also inserted into the hybrid aspen since poplar SLAC1 orthologs might have different unknown functions.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus tremuloides - Hybrid aspen
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Clone T89 and clone 51
Characteristics of the transformation process
Vector
pK7GWIWG2(II) and pGWB13 or pGWB40
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Slow Anion Channel-Associated 1 gene - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Tolerance to abiotic stress
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Hemagglutinin gene - Influenza virus - Influenza
Production of medical or pharmaceutical compounds (human or animal) - Vaccines
Enhanced Yellow Fluorescent Protein gene - Aequorea victoria - Crystal Jellyfish, Water Jellyfish, AEQVI
Selectable marker genes and reporter genes
Hygromycin B phosphotransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to antibiotics - Hygromycin
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
CaMV 35S terminator - Cauliflower mosaic virus - CaMV
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Notes regarding the genetic elements introduced or modified in this LMO
pK7GWIWG2(II) Vector
In this RNAi vector the expression of each of a AtSLAC1 ortholog and a AtSLAH3 ortholog from hybrid aspen is silenced. The silencing is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.

The vector confers kanamycin resistance by a nptII gene. The vector has also a chloramphenicol resistance gene from E. coli that is non-functional in plants.

pGWB13 or pGWB40 Vector
This vector expresses the A. thaliana SLAC1 gene in hybrid aspen. The AtSLAC1 expression is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.

To confirm SLAC1 expression in guard cells, the vector has either a hemagglutinin tag from the influenza virus or enhanced yellow fluorescence protein tag from Aequorea victoria).

The vector confers both hygromycin B resistance (by hpt gene) and kanamycin resistance (by nptII gene).
LMO characteristics
Modified traits
Common use(s)
  • Timber

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