Loading...
You are viewing a DELETED record.
This record information is displayed for reference purpose only and should be not used.
This document has been updated. This is not the latest published version. Click here to view the latest version of the record.
Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Hybrid aspen lines with modified stomatal regulation
EN
Multiple transformation events
No
-
Helsinki,Person:Division of Plant Biology and Institute of Biotechnology, Department of Biosciences
, FinlandPhone: +358 2941 57773,Fax:Email: jorma.vahala@helsinki.fi,Website:Related OrganizationUniversity of Helsinki ()Academic or research institute
The hybrid aspens were genetically modified to understand the function of stomatal regulation in hybrid aspen under natural growth conditions.
In these lines the function of stomata has been modified by silencing Slow Anion Channel-Associated 1 (SLAC1) and SLAC1 homolog 3 (SLAH3) orthologs of hybrid aspen under a guard-cell-specific promoter.
The A. thaliana SLAC1 gene is also inserted into the hybrid aspen since poplar SLAC1 orthologs might have different unknown functions.
EN
In these lines the function of stomata has been modified by silencing Slow Anion Channel-Associated 1 (SLAC1) and SLAC1 homolog 3 (SLAH3) orthologs of hybrid aspen under a guard-cell-specific promoter.
The A. thaliana SLAC1 gene is also inserted into the hybrid aspen since poplar SLAC1 orthologs might have different unknown functions.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
-
BCH-ORGA-SCBD-105731-2 Organism Populus tremula x Populus tremuloides (Hybrid aspen)Trees
Cultivar/breeding line: Clone T89 and clone 51
EN
pK7GWIWG2(II) and pGWB13 or pGWB40
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
-
BCH-GENE-SCBD-105733-1 Slow Anion Channel-Associated 1 gene | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Protein coding sequence | Tolerance to abiotic stress
-
BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
-
BCH-GENE-SCBD-45883-4 Hemagglutinin gene | Influenza A virus (Influenza, Flu, Avian flu, Human flu, Swine influenza, Equine influenza, Bird flu)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
-
BCH-GENE-SCBD-105732-1 Enhanced Yellow Fluorescent Protein gene | Aequorea victoria (Crystal Jellyfish, Water Jellyfish, AEQVI)Protein coding sequence | Selectable marker genes and reporter genes
-
BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
-
BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
-
BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
pK7GWIWG2(II) Vector
In this RNAi vector the expression of each of a AtSLAC1 ortholog and a AtSLAH3 ortholog from hybrid aspen is silenced. The silencing is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.
The vector confers kanamycin resistance by a nptII gene. The vector has also a chloramphenicol resistance gene from E. coli that is non-functional in plants.
pGWB13 or pGWB40 Vector
This vector expresses the A. thaliana SLAC1 gene in hybrid aspen. The AtSLAC1 expression is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.
To confirm SLAC1 expression in guard cells, the vector has either a hemagglutinin tag from the influenza virus or enhanced yellow fluorescence protein tag from Aequorea victoria).
The vector confers both hygromycin B resistance (by hpt gene) and kanamycin resistance (by nptII gene).
EN
In this RNAi vector the expression of each of a AtSLAC1 ortholog and a AtSLAH3 ortholog from hybrid aspen is silenced. The silencing is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.
The vector confers kanamycin resistance by a nptII gene. The vector has also a chloramphenicol resistance gene from E. coli that is non-functional in plants.
pGWB13 or pGWB40 Vector
This vector expresses the A. thaliana SLAC1 gene in hybrid aspen. The AtSLAC1 expression is regulated by one of two guard-cell-specific promoters, either the A. thaliana GC1 (At1g22690) ortholog from P. trichocarpa or the A. thaliana SLAC1 gene promoter.
To confirm SLAC1 expression in guard cells, the vector has either a hemagglutinin tag from the influenza virus or enhanced yellow fluorescence protein tag from Aequorea victoria).
The vector confers both hygromycin B resistance (by hpt gene) and kanamycin resistance (by nptII gene).
EN
- Timber
EN
EN
| Record type | Field | Record(s) | |||||
|---|---|---|---|---|---|---|---|
|
|||||||
