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Modified Organism
Hybrid aspen lines with modified wood development
Record information and status
Record ID
105737
Status
Published
Date of creation
2014-08-06 17:04 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-06 17:04 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Hybrid aspen lines with modified wood development
Transformation event
Multiple transformation events
Developer(s)
Division of Plant Biology and Institute of Biotechnology
Department of Biosciences
University of Helsinki
Helsinki
Finland
Phone:+358 2941 57773
Email:jorma.vahala@helsinki.fi
Description
Hybrid aspen were genetically modified to understand the role and function of plant hormones ethylene and cytokinin in wood formation.

In these lines the genetically modified hybrid aspens overexpress Ethylene Response Factor (ERF) genes or a gene enhancing cytokinin signaling under a constitutive LMX5 promoter, which directs the expression to wood forming tissues.

Overexpression of these genes modifies wood properties and enhances the growth.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Populus tremula x Populus tremuloides - Hybrid aspen
Point of collection or acquisition of the recipient organism
Cultivar/breeding line: Clone T89 and clone 51
Characteristics of the transformation process
Vector
LMX5
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Ethylene response factor gene - Populus trichocarpa - Western balsam poplar, Black cottonwood, California poplar, POPTR
Changes in physiology and/or production - Yield
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Gene 4 transcription terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Hygromycin B phosphotransferase gene - Streptomyces hygroscopicus - STRHY
Resistance to antibiotics - Hygromycin
Notes regarding the genetic elements introduced or modified in this LMO
LMX5 Vector
Three different ERF genes (ERF21, ERF30 and ERF33) from P. trichocarpa and a gene enhancing cytokinin response from A. thaliana are overexpressed.

The overexpressed genes are regulated by a constitutive LMX5 promoter from hybrid aspen.

The hpt gene conferring hygromycin B resistance. The vector has also ColE1 replicon and β-lactamase-gene for bacterial selection from Escherichia coli pBR322 plasmid that are non-functional in plants.
LMO characteristics
Modified traits
Common use(s)
  • Timber

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