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Modified Organism
SHD-30086-3 - Colour Modified Carnation
Record information and status
Record ID
105796
Status
Published
Date of creation
2014-08-14 14:51 UTC (jbocanegra@humboldt.org.co)
Date of publication
2014-08-21 14:44 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Colour Modified Carnation
Transformation event
30086
Unique identifier
SHD-30086-3
Developer(s)
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:+81 75 962 9132
Fax:+81 75 962 3791
Email:Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp
Description
Carnation variety 30086 flowers have a pastel violet/mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a pink coloured carnation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Characteristics of the transformation process
Vector
pCGP3601
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Chalcone synthase gene promoter - Antirrhinum majus - Common Snapdragon, Snapdragon
Flavonoid 3’, 5’-hydroxylase gene - Viola sp. - Pansy, VIOLA
Changes in quality and/or metabolite content - Pigmentation / Coloration
Acetohydroxy acid synthase gene - Nicotiana tabacum - Tobacco, TOBAC
Resistance to herbicides - Chlorsulfuron, Sulfonylurea
D8 gene terminator - Petunia hybrida - Petunia, PETHY
Anthocyanidin synthase gene promoter - Dianthus caryophyllus - Carnation, DIACA
Anthocyanin-3',5'-methyltransferase gene - Torenia sp. - Torenia hybrid, Wishbone flowers, Blue Wings, TOREN
Changes in quality and/or metabolite content - Pigmentation / Coloration, Flavonoids (e.g. anthocyanin)
Anthocyanidin synthase gene terminator - Dianthus caryophyllus - Carnation, DIACA
Dihydroflavonol-4-reductase - Dianthus caryophyllus - Carnation, DIACA
Changes in quality and/or metabolite content - Pigmentation / Coloration
Notes regarding the genetic elements introduced or modified in this LMO
The dihydroflavonol-4-reductase sequence from carnation is inserted in a sense/ antisense orientation. The transcription product results in the formation of a dsDFR which suppress the expression of the endogenous DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase thus leading to the synthesis of delphinidin imparting a violet/mauve colour to the carnation.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments which are modified by expression of an anthocyanin-3',5'-methyltransferase gene.

A Dihydroflavonol-4-reductase sequence from carnation has been utilised in a dsDFR format in order to suppress expression of the endogenous carnation DFR gene, thus allowing dominant expression of the introduced petunia Dihydroflavonol-4-reductase.

An anthocyanin-3',5'-methyltransferase gene has also been added. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce the anthocyanins petunidin-3-glucoside and malvidin-3-glucoside.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Additional Information
30086 was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing four genes that function together in the biosynthesis of the anthocyanin pigments related to delphinidin.

The transgenic lines were derived from the parent cultivar which is a pink coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr), a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and an anthocyanin-3',5'-methyltransferase gene.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Expression of a dsDFR construct suppresses the activity of endogenous DFR. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce  anthocyanins with a novel hue.