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Modified Organism
SYN-IR1Ø2-7 x MON-15985-7 - Bollgard® III
Record information and status
Record ID
105801
Status
Published
Date of creation
2014-08-15 17:19 UTC (cvela@cofepris.gob.mx)
Date of publication
2014-08-22 19:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Bollgard® III
Transformation event
COT102 x MON15985
Unique identifier
SYN-IR1Ø2-7 x MON-15985-7
Developer(s)
Monsanto
Description
The stacked cotton line SYN-IR1Ø2-7 x MON-15985-7 was obtained through the traditional cross breading of each of the parental organisms to produce a cotton that expresses each of Vip3A, Cry2Ab2, and Cry1Ac  genes. The expression of these genes are expected to confer resistance to Lepidoptera.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
SYN-IR1Ø2-7 - VIPCOT™ Cotton
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pCOT-1 and PV-GHBK11 (PV-GHBK04 pre-existing in parental organism)
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
HSP 70 5' untranslated leader sequence
0.10 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
Cry2Ab2
1.91 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Cry1Ac
0.35 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.63 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.79 Kb
 
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Beta-Glucuronidase coding sequence
1.81 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Actin 2 Gene Promoter
0.00 Kb
 
 
Vegetative insecticidal protein 3A
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene 3 promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from COT102 vector pCOT-1:
Cotton tolerant to lepidopteran pests through introduction of the vip3A(a) gene which codes for an insecticidal protein that targets lepidopteran insect species. The aph4 gene, coding for hygromycin-B phosphotransferase (APH4) was used as a selectable marker.

DNA insert from MON-15985-7 vectors PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Risk Assessment2 records