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Modified Organism
SHD-27531-4 - Moontea™ carnation
Record information and status
Record ID
105802
Status
Published
Date of creation
2014-08-21 21:36 UTC (jbocanegra@humboldt.org.co)
Date of last update
2020-11-23 15:20 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-11-23 15:20 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moontea™ carnation
Transformation event
27531
Unique identifier
SHD-27531-4
Developer(s)
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:+81 75 962 9132
Fax:+81 75 962 3791
Email:Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp
Description
Carnation variety 27531 flowers have a mauve colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The transgenic lines were derived from the parent cultivar which is a cream coloured carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of coloured delphinidin anthocyanin pigments. The modified flowers additionally contain tobacco acetolactate synthase for chlorsulfuron herbicide tolerance.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Related LMOs
FLO-11363-2 - Moonshadow™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø689-6 - Moonaqua™ carnation
Dr Yoshikazu Tanaka Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø685-2 - Moonvista™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Show detection method(s)
Characteristics of the transformation process
Vector
pCGP1991
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.20 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
3.77 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Dihydroflavonol-4-reductase promoter
0.00 Kb
 
 
Dihydroflavonol-4-reductase
4.96 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.17 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.80 Kb
 
 
D8 gene terminator
0.81 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
Three gene cassettes are present: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida dihydroflavonol-4-reductase (DFR) and Viola sp. flavonoid3', 5'-hydroxylase (F3'5'H).

Transcription of ALS is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and a N. tabacum ALS terminator. A 5' untranslated leader sequence from P. hybrida chlorophyll a/b-binding protein is also present at the 5' end of ALS, but is not expected to be translated. The leader sequence promotes high levels of transcription of ALS.

Transcription of DFR is under control of its endogenous promoter and terminator. The coding sequence contains 6 exons and 5 introns.

Transcription of F3'5'H is under control of an Antirrhinum majus chalcone synthase promoter and a P. hybrida D8 terminator.

Note:
- The size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of ALS coding sequence + ALS terminator)
- The size of the DFR coding sequence represents the size of the full genomic cone (4.96 kb = DFR promoter + DFR coding sequence + DFR terminator)
- The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Ornamental

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Risk Assessment3 records