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Modified Organism
SHD-29952-4 - Colour modified carnation
Record information and status
Record ID
105853
Status
Published
Date of creation
2014-08-28 14:18 UTC (jbocanegra@humboldt.org.co)
Date of publication
2014-09-03 16:59 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Colour modified carnation
Transformation event
29952
Unique identifier
SHD-29952-4
Developer(s)
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:+81 75 962 9132
Fax:+81 75 962 3791
Email:Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp
Description
Carnation variety 29952 flowers have a novel flower colour due to the biosynthesis of the anthocyanin pigment delphinidin. This pigment is not produced in non-transgenic carnation. The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) from petunia and a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H). Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Acetohydroxy acid synthase gene - Nicotiana tabacum - Tobacco, TOBAC
Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Chalcone synthase gene promoter - Antirrhinum majus - Common Snapdragon, Snapdragon
Flavonoid 3’, 5’-hydroxylase gene - Viola sp. - Pansy, VIOLA
Changes in quality and/or metabolite content - Pigmentation / Coloration
D8 gene terminator - Petunia hybrida - Petunia, PETHY
Anthocyanidin synthase gene promoter - Dianthus caryophyllus - Carnation, DIACA
Anthocyanin-3',5'-methyltransferase gene - Torenia sp. - Torenia hybrid, Wishbone flowers, Blue Wings, TOREN
Changes in quality and/or metabolite content - Pigmentation / Coloration, Flavonoids (e.g. anthocyanin)
Anthocyanidin synthase gene terminator - Dianthus caryophyllus - Carnation, DIACA
CaMV Enhanced 35S promoter - Cauliflower mosaic virus - CaMV
Flavone synthase gene - Torenia sp. - Torenia hybrid, Wishbone flowers, Blue Wings, TOREN
Changes in quality and/or metabolite content - Pigmentation / Coloration, Flavonoids (e.g. anthocyanin)
Notes regarding the genetic elements introduced or modified in this LMO
29952 was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing four genes that function together in the biosynthesis of the anthocyanin pigments related to delphinidin.

The genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr), a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), an anthocyanin-3',5'-methyltransferase gene and a flavone synthase gene.

Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations. Anthocyanin-3',5'-methyltransferase (AMT) methylates delphinidin-3-glucoside to produce  anthocyanins with a novel hue. Production of flavones increases the blue hue of the flower colour.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Other relevant website address or attached documents