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Modified Organism
Insect resistent rice
Record information and status
Record ID
106014
Status
Published
Date of creation
2014-10-30 10:33 UTC (enrico.ben@ec.europa.eu)
Date of publication
2014-11-03 18:23 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistent rice
Transformation event
Bt63 (TT51-1)
Developer(s)
Huazhong Agricultural University
Shizishan Street N. 1
Hongshan District
Wuhan, Hubei
China, 430070
Phone:+86 27 87281181
Fax:+86 27 87396057
Description
Transgenic rice BT63 (TT51-1) is an insect-resistant strain including a codon optimized, truncated Cry1Ab/Ac cassette derived from sequences of Bacillus thuringiensis subsp. Kurstaki HD1 and HD73.

The synthetic construct encodes an insecticidal protein conferring resistance against certain lepidoptera species.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Characteristics of the transformation process
Vector
pFHBT1 and pGL2RC7
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Rice actin 1 gene promoter
0.00 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Cry1Ab/Ac
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
In the cassette the hybrid Cry1Ab/Ac gene is under the control of the rice Actin 1 promoter with its first intron and of the Nos terminator.

The transgenic insert contains two tandem repeats of a Cry1Ab/Ac cassette integrated at the same site as a head-to tail concatemer.

The first Cry1Ab/Ac cassette at the 5' end of the transgenic insert contains a truncated Actin 1 promoter (149 bp) which is missing the regulatory elements for maximal gene expression and cannot encode for a functional protein.

The second Cry1Ab/Ac cassette is complete. An 83 bp long segment of the Amp gene is located between the two tandem repeats of the Cry1Ab/Ac cassette. Incomplete ampicillin (653 bp) and hygromycin (50 bp) resistance genes are further located in the 3' downstream region.
LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication3 records
Organization1 record