Record details

Living Modified Organism identity

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LMO name

Insect resistent rice

Transformation event

Bt63 (TT51-1)

Developer(s)

Record #106013

Huazhong Agricultural University

Shizishan Street N. 1
Hongshan District

Wuhan, Hubei

China, 430070

Phone:	+86 27 87281181
Fax:	+86 27 87396057

Description

Transgenic rice BT63 (TT51-1) is an insect-resistant strain including a codon optimized, truncated Cry1Ab/Ac cassette derived from sequences of Bacillus thuringiensis subsp. Kurstaki HD1 and HD73.

The synthetic construct encodes an insecticidal protein conferring resistance against certain lepidoptera species.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #10454

Oryza sativa - Rice, ORYSA

Characteristics of the transformation process

Vector

pFHBT1 and pGL2RC7

Techniques used for the modification

Biolistic / Particle gun

Genetic elements construct

Rice actin 1 gene promoter

#100364

0.00 Kb

Rice actin 1, intron

#100355

0.00 Kb

Cry1Ab/Ac

#103109

0.00 Kb

Nopaline Synthase Gene Terminator

#100269

0.00 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

In the cassette the hybrid Cry1Ab/Ac gene is under the control of the rice Actin 1 promoter with its first intron and of the Nos terminator.

The transgenic insert contains two tandem repeats of a Cry1Ab/Ac cassette integrated at the same site as a head-to tail concatemer.

The first Cry1Ab/Ac cassette at the 5' end of the transgenic insert contains a truncated Actin 1 promoter (149 bp) which is missing the regulatory elements for maximal gene expression and cannot encode for a functional protein.

The second Cry1Ab/Ac cassette is complete. An 83 bp long segment of the Amp gene is located between the two tandem repeats of the Cry1Ab/Ac cassette. Incomplete ampicillin (653 bp) and hygromycin (50 bp) resistance genes are further located in the 3' downstream region.

LMO characteristics

Modified traits