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LMOs, Genes or Organisms
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Survey on indicators (2014)
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Insect resistent rice
Decisions on the LMO
Record information and status
Date of creation
2014-10-30 10:33 UTC (firstname.lastname@example.org/14451)
Date of publication
2014-11-03 18:23 UTC (email@example.com)
Living Modified Organism identity
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Insect resistent rice
Huazhong Agricultural University
Shizishan Street N. 1
+86 27 87281181
+86 27 87396057
Transgenic rice BT63 (TT51-1) is an insect-resistant strain including a codon optimized, truncated Cry1Ab/Ac cassette derived from sequences of Bacillus thuringiensis subsp. Kurstaki HD1 and HD73.
The synthetic construct encodes an insecticidal protein conferring resistance against certain lepidoptera species.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Characteristics of the transformation process
pFHBT1 and pGL2RC7
Techniques used for the modification
Biolistic / Particle gun
Genetic elements construct
Rice actin 1 gene promoter
Rice actin 1, intron
Nopaline Synthase Gene Terminator
Notes regarding the genetic elements introduced or modified in this LMO
In the cassette the hybrid Cry1Ab/Ac gene is under the control of the rice Actin 1 promoter with its first intron and of the Nos terminator.
The transgenic insert contains two tandem repeats of a Cry1Ab/Ac cassette integrated at the same site as a head-to tail concatemer.
The first Cry1Ab/Ac cassette at the 5' end of the transgenic insert contains a truncated Actin 1 promoter (149 bp) which is missing the regulatory elements for maximal gene expression and cannot encode for a functional protein.
The second Cry1Ab/Ac cassette is complete. An 83 bp long segment of the Amp gene is located between the two tandem repeats of the Cry1Ab/Ac cassette. Incomplete ampicillin (653 bp) and hygromycin (50 bp) resistance genes are further located in the 3' downstream region.
Resistance to diseases and pests
Lepidoptera (butterflies and moths)
Page dedicated to detection of "Bt63" rice on the EURL-GMFF (European Union Reference Laboratory for GM Food and Feed) website
Bt63 detection method on GMOMETHODS: the EU Database of Reference Methods for GMO Analysis
Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection - Journal of Agricultural and Food Chemistry (ACS Publications).htm
Detection of genetically modified rice collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice - Springer.htm
Other relevant website address or attached documents
Characterization of the Transgenic Rice Event TT51–1 and Construction of a Reference Plasmid - Journal of Agricultural and Food Chemistry (ACS Publications).htm
The first approved transgenic rice in China
Expression and Function of a Hybrid Bt Toxin Gene in Transgenic Rice Conferring Resistance to Insect Pest
Records referencing this document
Country's Decision or any other Communication
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