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Modified Organism
Canola modified for herbicide tolerance
Record information and status
Record ID
106260
Status
Published
Date of creation
2014-12-20 11:30 UTC (g.mozgova@igc.by)
Date of last update
2015-01-09 08:15 UTC (g.mozgova@igc.by)
Date of publication
2015-01-09 16:19 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Canola modified for herbicide tolerance
Transformation event
aroA
Developer(s)
Анатолий Николаевич Евтушенков
Head of the Department of Molecular Biology
Department of Molecular Biology
Faculty of Biology of the Belarusian State University
Kurchatova, 10
Minsk
Belarus
Phone:+375 17 2095898
Email:evtushenkov@bsu.by
Description
Transgenic rapeseed line with an integrated, modified, aroA gene to confer resistance to the herbicide glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Characteristics of the transformation process
Vector
pBI121
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Nopaline Synthase Gene Promoter - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
CaMV 35S promoter - Cauliflower mosaic virus - CaMV
Nopaline Synthase Gene Terminator - Agrobacterium tumefaciens - Agrobacterium, Rhizobium, RHIRD
5-enolpyruvylshikimate-3-phosphate synthase gene - Dickeya dadantii - DICDA
Resistance to herbicides - Glyphosate
Chloroplast Transit Peptide - Nicotiana tabacum - Tobacco, TOBAC
Notes regarding the genetic elements introduced or modified in this LMO
Transgenic rapeseed line with integrated gene aroA under control of the constitutive 35S promoter of cauliflower mosaic virus and nopaline synthase termination sequence of A. tumefaciens. Incorporation of the aroA target gene confers resistance to the herbicide glyphosate.  To increase transgenic line resistance  to glyphosate single nucleotide mutation was introduced into the aroA sequence by the site-directed mutagenesis.
LMO characteristics
Modified traits

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism1 record
Risk Assessment1 record