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Modified Organism
UFL-X17CP-9 - Papaya modified for resistance to Papaya Ringspot Virus
Record information and status
Record ID
106312
Status
Published
Date of creation
2015-01-26 23:45 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 23:45 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Papaya modified for resistance to Papaya Ringspot Virus
Transformation event
X17-2
Unique identifier
UFL-X17CP-9
Developer(s)
Michael J. Davis
Professor, Institute of Food and Agricultural Sciences
Citrus Research and Education Center
University of Florida
700 Experiment Station Road
Lake Alfred, Florida
United States of America, 33850-1299
Description
Transgenic papaya modified to express the coat protein (CP) gene of Hawaiian PRSV isolate HA5-1 thereby conferring resistance to the Papaya Ringspot Virus.

NOTE: This LMO was formerly referred to with the UID UFL-X17CP-6
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Carica papaya - Papaya, Pawpaw, Papaw, CARPA
Characteristics of the transformation process
Vector
pBI121fs
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
PRSV coat protein
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A uidA leader sequence from E.Coli is inserted after the CaMV 35S promoter to enhance the expression of PRSV CP in papaya.

The developer reports that the insert in line X17-2 has persisted as a single copy through six generations of progeny following the initial transformation and that no vector backbone sequence is present, as determined by Southern blot. The developer also notes that PCR analysis of the 5th generation progeny of the original transformant reveals the elimination of the frame shift in the prsv-cp gene, and that low level expression of PRSV-CP is detectable by Western blot (FDA).
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents