PPO Suppression Transgene | Malus domestica (Apple, MALDO) | BCH-GENE-SCBD-106414 | Genetic element | Biosafety Clearing-House

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Genetic element (GENE)
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published: 20 Feb 2015 last updated: 30 Jul 2019
General information
PPO Suppression Transgene
EN
  • PGAS
    EN
CS-PGAS-SYNTH
EN
Double-stranded RNA
Yes
Donor organism
EN
Additional Information
Polyphenol Oxidase (PPO), responsible for apple browning, is encoded by a diverse, multi-gene family whose members are expressed both in early fruit development and in response to wounding. The suppression of PPO, which is expected to lead to a nonbrowning phenotype, requires the use of a chimeric suppression transgene containing chosen sequences from the PPO gene family.

The PPO suppression transgene (PGAS) consists of 394, 457, 457 and 453 bp regions of apple PPO genes (PPO2, GPO3, APO5, pSR7, respectively), placed in the sense orientation. Suppression is achieved through the formation of dsRNA between the suppression transcript and native PPO mRNA. This is subsequently processed into small interfering RNAs (siRNAs) that direct the cleavage of the target mRNA.

The transgene is designed to reduce overall expression of the entire apple PPO gene family, and to induce a reduced browning or nonbrowning phenotype in apple.
EN
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Record type Field Record(s)
Living Modified Organism Introduced or modified genetic element(s) 3
Living Modified Organism Genetic elements construct 3