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Gene and DNA Sequence
Record information and status
Record ID
106414
Status
Published
Date of creation
2015-02-20 15:44 UTC (dina.abdelhakim@cbd.int)
Date of last update
2015-02-20 16:43 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-02-20 16:43 UTC (dina.abdelhakim@cbd.int)

General information
Name of the gene or DNA sequence
PPO Suppression Transgene
Alternate Genetic Element Name (synonyms)
PGAS
Abbreviation
CS-PGAS-SYNTH
Category of DNA sequence
  • Double-stranded RNA
Is the gene / DNA sequence is a synthetic molecule?
Yes
Donor organism
Donor organism
Malus domestica - Apple, MALDO
Additional Information
Additional Information
Polyphenol Oxidase (PPO), responsible for apple browning, is encoded by a diverse, multi-gene family whose members are expressed both in early fruit development and in response to wounding. The suppression of PPO, which is expected to lead to a nonbrowning phenotype, requires the use of a chimeric suppression transgene containing chosen sequences from the PPO gene family.

The PPO suppression transgene (PGAS) consists of 394, 457, 457 and 453 bp regions of apple PPO genes (PPO2, GPO3, APO5, pSR7, respectively), placed in the sense orientation. Suppression is achieved through the formation of dsRNA between the suppression transcript and native PPO mRNA. This is subsequently processed into small interfering RNAs (siRNAs) that direct the cleavage of the target mRNA.

The transgene is designed to reduce overall expression of the entire apple PPO gene family, and to induce a reduced browning or nonbrowning phenotype in apple.

Records referencing this document (2)
IDDescription
2record(s) found
Modified Organism2 records