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Modified Organism
OKA-NBØØ1-8 - Arctic™ "Golden Delicious" Apple
Record information and status
Record ID
106416
Status
Published
Date of creation
2015-02-20 15:59 UTC (dina.abdelhakim@cbd.int)
Date of last update
2015-02-20 16:51 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-02-20 16:52 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Arctic™ "Golden Delicious" Apple
Transformation event
GD743
Unique identifier
OKA-NBØØ1-8
Developer(s)
Neal Carter
Okanagan Specialty Fruits Inc.
PO Box 1533
Summerland, BC
Canada, V0H 1Z0
Description
Arctic Apple are modified to resist to enzymatic browning  by inserting a chimeric polyphenol oxidase (PPO) suppression element derived from apple PPO sequences. The flesh of modified apples subjected to mechanical damage, such as slicing or bruising, does not brown as compared to a conventional apple.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Malus domestica - Apple, MALDO
Point of collection or acquisition of the recipient organism
Golden Delicious
Related LMOs
OKA-NBØØ2-9 - Arctic™ "Granny Smith" Apple
Neal Carter Resistance to antibiotics - Kanamycin
Characteristics of the transformation process
Vector
GEN-03
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.65 Kb
 
 
5' Untranslated Leader of AMV RNA4
0.00 Kb
 
 
PPO Suppression Transgene
1.81 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Nopaline Synthase Gene Promoter
0.31 Kb
 
 
Neomycin Phosphotransferase II
0.98 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
PCR and southern blot analysis indicate that the presence of both the 3' end of the PPO suppression transgene and the NptII selection marker as well as the presence of an intact copy of the PPO suppression portion of the insert.

Southern data, combined with Mendelian Inheritance data indicates that GD743 contains two unlinked insertions. Southern blots also indicates that there is no evidence for inclusion of a complete copy of the vector backbone in the modified genome.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Other relevant website address or attached documents

Records referencing this document (1)
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