| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Silver birch lines with potential pest or fungal disease resistence
Record information and status
Record ID
107612
Status
Published
Date of creation
2015-03-09 11:38 UTC (gtlk.stm@stm.fi)
Date of publication
2015-03-09 16:23 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Silver birch lines with potential pest or fungal disease resistence 
Transformation event
5 different transformation events
Developer(s)
von Weissenberg
Faculty of Agriculture and Forestry Department of Plant Biology
University of Helsinki
Helsinki
Finland
Phone:+358 2941 911
Email:kim.vonweissenberg@helsinki.fi
Description
Silver  birch lines expressing different combinations of 5 different gene constructs containing chitinase, glucanase or proteinase inhibitor genes with a view to investigate the resistance to diseases and insects in birch
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Betula pendula - Silver birch, European white birch, Warty birch, BETPN
Point of collection or acquisition of the recipient organism
Jr1/4
Characteristics of the transformation process
Vector
Various
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Chitinase coding sequence - Beta vulgaris - Common beet, Sugarbeet, BETMA
Resistance to diseases and pests - Fungi
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Glucanase coding sequence - Beta vulgaris - Common beet, Sugarbeet, BETMA
Resistance to diseases and pests - Fungi
Proteinase inhibitor II gene - Solanum tuberosum - Potato, SOLTU
Resistance to diseases and pests
Wound induceable promoter - Solanum tuberosum - Potato, SOLTU
Peroxidase coding sequence - Picea abies - Norway spruce, PICAB
Resistance to diseases and pests - Fungi
Beta-Glucuronidase coding sequence - Escherichia coli - ECOLX
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
Description of each of the transformation events:

1) Chitinase gene from sugar beet in pBKL4. Potential fungal resistance gene. The enzyme hydrolyses chitin which is an element in fungal cell walls. Construct contains uidA and nptII gene, which are also transformed into plant.

2) Chitinase and glucanase genes from sugar beet in pBKL4 Seq. Potential fungal resistance genes. These enzymes hydrolyse chitin and glucans, respectively, which are elements in fungal cell walls. Construct contains uidA and nptII gene which are also transformed into plant.

3) Proteinase inhibitor gene from potato in pRT104. Potential insect resistance gene. The gene inhibits serine proteinase activities. Construct contains nptII gene, which are also transformed into plant.

4) Wound induced promoter from potato in pRT210. Promoter, which is shown to function systemically in birch controls uidA gene. To study the stability of gene expression. Construct also contains nptII gene.

5) Peroxidase gene fragment from spuruce in antisense orientation in pPCV7O2.SPI2.5. Potential lignin modification enzyme. Construct contains nptII gene.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (1)
IDDescription
1record(s) found
Risk Assessment1 record