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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Silver birch lines with potential pest or fungal disease resistence
EN
5 different transformation events
No
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Helsinki,Person:von Weissenberg, Faculty of Agriculture and Forestry Department of Plant Biology
, FinlandPhone: +358 2941 911,Fax:Email: kim.vonweissenberg@helsinki.fi,Website:Related OrganizationUniversity of Helsinki ()Academic or research institute
Silver birch lines expressing different combinations of 5 different gene constructs containing chitinase, glucanase or proteinase inhibitor genes with a view to investigate the resistance to diseases and insects in birch
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-106324-2 Organism Betula pendula (Silver birch, European white birch, Warty birch, BETPN)Trees
Jr1/4
EN
Various
EN
- Agrobacterium-mediated DNA transfer
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-106332-1 Chitinase coding sequence | Beta vulgaris (Common beet, Sugarbeet, BETMA)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-106343-1 Glucanase coding sequence | Beta vulgaris (Common beet, Sugarbeet, BETMA)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-104338-1 Proteinase inhibitor II gene | Solanum tuberosum (Potato, SOLTU)Protein coding sequence | Resistance to diseases and pests
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BCH-GENE-SCBD-106342-1 Wound induceable promoter | Solanum tuberosum (Potato, SOLTU)Promoter
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BCH-GENE-SCBD-106344-1 Peroxidase coding sequence | Picea abies (Norway spruce, PICAB)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
Description of each of the transformation events:
1) Chitinase gene from sugar beet in pBKL4. Potential fungal resistance gene. The enzyme hydrolyses chitin which is an element in fungal cell walls. Construct contains uidA and nptII gene, which are also transformed into plant.
2) Chitinase and glucanase genes from sugar beet in pBKL4 Seq. Potential fungal resistance genes. These enzymes hydrolyse chitin and glucans, respectively, which are elements in fungal cell walls. Construct contains uidA and nptII gene which are also transformed into plant.
3) Proteinase inhibitor gene from potato in pRT104. Potential insect resistance gene. The gene inhibits serine proteinase activities. Construct contains nptII gene, which are also transformed into plant.
4) Wound induced promoter from potato in pRT210. Promoter, which is shown to function systemically in birch controls uidA gene. To study the stability of gene expression. Construct also contains nptII gene.
5) Peroxidase gene fragment from spuruce in antisense orientation in pPCV7O2.SPI2.5. Potential lignin modification enzyme. Construct contains nptII gene.
EN
1) Chitinase gene from sugar beet in pBKL4. Potential fungal resistance gene. The enzyme hydrolyses chitin which is an element in fungal cell walls. Construct contains uidA and nptII gene, which are also transformed into plant.
2) Chitinase and glucanase genes from sugar beet in pBKL4 Seq. Potential fungal resistance genes. These enzymes hydrolyse chitin and glucans, respectively, which are elements in fungal cell walls. Construct contains uidA and nptII gene which are also transformed into plant.
3) Proteinase inhibitor gene from potato in pRT104. Potential insect resistance gene. The gene inhibits serine proteinase activities. Construct contains nptII gene, which are also transformed into plant.
4) Wound induced promoter from potato in pRT210. Promoter, which is shown to function systemically in birch controls uidA gene. To study the stability of gene expression. Construct also contains nptII gene.
5) Peroxidase gene fragment from spuruce in antisense orientation in pPCV7O2.SPI2.5. Potential lignin modification enzyme. Construct contains nptII gene.
EN
- Research
EN
EN
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