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Modified Organism
Rhodococcus equi Vaccine
Record information and status
Record ID
107877
Status
Published
Date of creation
2015-03-24 07:46 UTC (german_bch@bvl.bund.de)
Date of publication
2015-04-13 20:53 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rhodococcus equi Vaccine
Transformation event
Rhodococcus equi RG2837, R. equi ΔipdABΔipdAB2
Developer(s)
Intervet
Intervet International B.V.
Wim de Körverstraat 35
Boxmeer
Netherlands, 5831 AN
Phone:+ 31 48 558 7600
Email:animal-health-communications@merck.com
Url:MERCK Animal Health
Description
Rhodococcus equi RG2837 is a double-deletion mutant of the R. equi wild-type RE1. The original strain possesses the virulence plasmid VapA and triggers the typical clinical signs of respiratory diseases (pneumonia) in foals after infection. Although the LMO R. equi RG2837 also has the VapA plasmid, four introduced deletions in the bacterial chromosome prevent survival and proliferation of R. equi RG2837 in macrophages. Since this is a requirement for manifestation of an infection the deletion mutant is suitable to be used as a live-attenuated vaccine.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Rhodococcus equi - RHOE1
Characteristics of the transformation process
Vector
pSelAct-ipd1 and pSelAct-ΔipdAB2
Techniques used for the modification
  • Electroporation
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
CoA-transferase alpha subunit gene - Rhodococcus equi - RHOE1
Resistance to diseases and pests - Bacteria
CoA-transferase beta subunit gene - Rhodococcus equi - RHOE1
Resistance to diseases and pests - Bacteria
CoA-transferase alpha 2 subunit gene - Rhodococcus equi - RHOE1
Resistance to diseases and pests - Bacteria
CoA-transferase beta 2 subunit gene - Rhodococcus equi - RHOE1
Resistance to diseases and pests - Bacteria
Notes regarding the genetic elements introduced or modified in this LMO
LMO R. equi RG2837 differs from its wild-type strain R. equi RE1 by the absence of most of the coding sequences of chromosomal genes ipdA, ipdB, ipdA2 and ipdB2 - genes which are involved in the cholesterol catabolism. Due to genetic engineering two recognition sites for restriction enzymes of 6 bp in length from the vectors pSelAct-ipd1 and pSelAct-ΔipdAB2 remained in the bacterial chromosome of RG2837.
LMO characteristics
Modified traits
  • vaccine
Common use(s)
  • Vaccine

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record