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Modified Organism
Maize with altered growth and tolerance to herbicides
Record information and status
Record ID
107960
Status
Published
Date of creation
2015-04-28 08:00 UTC (didier.breyer@wiv-isp.be)
Date of last update
2019-08-20 18:28 UTC (austein.mcloughlin@cbd.int)
Date of publication
2019-08-20 18:28 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize with altered growth and tolerance to herbicides
Transformation event
Zea Mays B104(GA2ox::KLUH) x CML91
Developer(s)
Dr. Hilde Nelissen
Departement Plantensysteembiologie
VIB - Universiteit Gent (VIB-UGent)
Technologiepark 927
Gent
Belgium, B-9052
Phone:+32 9 2446611
Email:vib@vib.be
Url:VIB
Description
The genetically modified maize plants have modified growth characteristics resulting from the additional expression of the CYP78A1 gene (also called the KLUH  or PLA1 gene) under the control of the GA2oxidase promoter.

The CYP78A1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification the plants have a little more robust appearance, but above all significantly larger leaves. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval. The modified maize demonstrates longer cell division intervals, which compensate for abiotic stress, such as cold and drought.

Additionally the bar-gene from Streptomyces hygroscopicus is present in the plant as a selection marker gene. This bar-gene produces the Phosphinotricin Acetyl Transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: inbred line B104
Related LMOs
Maize with altered growth and tolerance to herbicides
Prof. Dr. Frank Van Breusegem Changes in physiology and/or production - Growth rate Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
pBbm42GW7
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
GA2-oxidase promoter
2.05 Kb
 
 
Plastochron 1
1.83 Kb
 
 
CaMV 35S terminator
0.27 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Omega 5' untranslated leader
0.09 Kb
 
 
CaMV 35S promoter
0.94 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transcription of the Zea mays (maize) plastochron1 (ZmPla1) gene commences from the maize GA2-oxidase (GA2ox) promoter and terminates at the Cauliflower Mosaic Virus (CaMV) 35S terminator. The transcribed sequence of ZmPla1 was derived from the reverse transcription of the messenger RNA for ZmPLA1 (cDNA). Thus, no introns are present in the coding sequence that was integrated into the genome.

In the other gene cassette, transcription of the Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar) gene starts from the CaMV 35S promoter and ends at the Agrobacterium tumefaciens nopaline synthase terminator. The transcript contains a 5' untranslated Tobacco Mosaic Virus omega leader sequence to enhance translation of the bar gene.
LMO characteristics
Modified traits
Other gene(s) whose expression was affected by the transformation
Plastochron 1 - Zea mays - Maize, Corn, MAIZE
Changes in physiology and/or production - Yield, Growth rate, Reproduction
Tolerance to abiotic stress - Cold / Heat, Drought
How the expression of the gene(s) was affected
The expression of the ZmPla1 occurs from two sources: the native, endogenous gene and the integrated gene cassette. Thus, it is expected that an elevated level of transcript and protein would result compared to a non-modified maize.
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record