| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Maize with altered growth and tolerance to herbicides
Record information and status
Record ID
107960
Status
Published
Date of creation
2015-04-28 08:00 UTC (didier.breyer@wiv-isp.be)
Date of publication
2015-04-29 18:22 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize with altered growth and tolerance to herbicides
Transformation event
Zea Mays B104(GA2ox::KLUH) x CML91
Developer(s)
Dr. Hilde Nelissen
Departement Plantensysteembiologie
VIB - Universiteit Gent (VIB-UGent)
Technologiepark 927
Gent
Belgium, B-9052
Phone:+32 9 2446611
Email:vib@vib.be
Url:VIB
Description
The genetically modified maize plants have modified growth characteristics resulting from the additional expression of the CYP78A1 gene (also called the 'KLUH' gene) under the control of the GA2oxidase promoter.

The CYP78A1 gene codes for a cytochrome P450 mono-oxygenase enzyme that is involved in the production of factors that control cell proliferation. As a result of the modification the plants have a little more robust appearance, but above all significantly larger leaves. The modification also results in more branches being formed on the tassel and a shorter anthesis-silking interval.

Additionally the bar-gene from Streptomyces hygroscopicus is present in the plant as a selection marker gene. This bar-gene produces the Phosphinotricin Acetyl Transferase enzyme (PAT), which acetylates phosphinotricin, also known as glufosinate, the active ingredient of herbicides such as Basta and Liberty.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
Cultivar/ Breeding line: inbred line B104
Related LMOs
Maize with altered growth and tolerance to herbicides
Prof. Dr. Frank Van Breusegem Changes in physiology and/or production - Growth rate Resistance to herbicides - Glufosinate
Characteristics of the transformation process
Vector
Agrobacterium Ti plasmid
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
AttB λ attachment site
0.00 Kb
 
 
GA2-oxidase promoter
0.00 Kb
 
 
AttB λ attachment site
0.00 Kb
 
 
Cytochrome P450 mono-oxygenase gene
0.00 Kb
 
 
AttB λ attachment site
0.00 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The LMO contains 3 mutated versions of the AttB gene that was originally isolated from E.coli. The genes are designated as Attb4, AttB1 and AttB2. They are ordered as stated within the construct.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record