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Biosafety Virtual Library Resources
(VLR)
last updated: 08 May 2015
Biosafety and Detection of Genetically Modified Organisms (Chapter 21 in Transgenic Plants - Advances and Limitations)
EN
InTech
EN
2012-05
EN
Transgenic Plants - Advances and Limitations (Edited by Yelda Özden Çiftçi)
EN
Chapters published March 07, 2012 under CC BY 3.0 license
EN
This Chapter is divided into the following sections:
1. Introduction
2. Transgenic plants and their advantages
3. Food biosafety
4. Environmental biosafety
5. Biosafety regulations
5.1 Regulatory agencies
5.2 Other recently developed techniques to avoid environmental and human health concerns
5.2.1 Chloroplast engineering
5.2.2 Producing marker-free transgenic plants
6. Detection of genetically modified organisms
6.1 Techniques for GMO detection
6.1.1 Direct assays: Detection of the presence of exogenous DNA
6.1.1.1 PCR
6.1.1.2 Real-time PCR
6.1.1.3 Southern blotting
6.1.2 Indirect assays: Detection based on the presence of RNA
6.1.2.1 Northern blotting
6.1.2.2 RT-PCR
6.1.3 Indirect assays: Detection based on the presence of protein
6.1.3.1 Bioassays
6.1.3.2 Immunoassays
6.1.4 Alternative techniques for the detection of GMOs
7. Conclusions
8. Acknowledgements
9. References
EN
1. Introduction
2. Transgenic plants and their advantages
3. Food biosafety
4. Environmental biosafety
5. Biosafety regulations
5.1 Regulatory agencies
5.2 Other recently developed techniques to avoid environmental and human health concerns
5.2.1 Chloroplast engineering
5.2.2 Producing marker-free transgenic plants
6. Detection of genetically modified organisms
6.1 Techniques for GMO detection
6.1.1 Direct assays: Detection of the presence of exogenous DNA
6.1.1.1 PCR
6.1.1.2 Real-time PCR
6.1.1.3 Southern blotting
6.1.2 Indirect assays: Detection based on the presence of RNA
6.1.2.1 Northern blotting
6.1.2.2 RT-PCR
6.1.3 Indirect assays: Detection based on the presence of protein
6.1.3.1 Bioassays
6.1.3.2 Immunoassays
6.1.4 Alternative techniques for the detection of GMOs
7. Conclusions
8. Acknowledgements
9. References
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No
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Identifier (ISBN, ISSN, etc.)
ISBN 978-953-51-0181-9; DOI: 10.5772/1409
Format
23-page PDF file