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Modified Organism
Canola modified for herbicide tolerance
Record information and status
Record ID
108048
Status
Published
Date of creation
2015-05-15 08:58 UTC (g.mozgova@igc.by)
Date of publication
2015-05-26 21:00 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Canola modified for herbicide tolerance
Transformation event
aroA, thiO
Developer(s)
Анатолий Николаевич Евтушенков
Head of the Department of Molecular Biology
Department of Molecular Biology
Faculty of Biology of the Belarusian State University
Kurchatova, 10
Minsk
Belarus
Phone:+375 17 2095898
Email:evtushenkov@bsu.by
Description
Canola was modified with the insertion of the aroA gene from Dickeya dadantii encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and the thiO gene from Bacillus subtilis which encodes a glycine oxidase. Te transformation of these genes into the canola genome confers resistance to the herbicide glyphosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
Canola modified for herbicide tolerance
Анатолий Николаевич Евтушенков Resistance to antibiotics - Kanamycin Resistance to herbicides - Glyphosate
Characteristics of the transformation process
Vector
pZH513 constructed from the pGreen vector by inserting the Dickeya dadantii aroA gene and the Bacillus subtilis thiO gene into the T-region
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
5-enolpyruvylshikimate-3-phosphate synthase gene - Dickeya dadantii - DICDA
Resistance to herbicides - Glyphosate
Glycine Oxidase gene - Bacillus subtilis - Bacillus, BACIU
Resistance to herbicides - Glyphosate
Notes regarding the genetic elements introduced or modified in this LMO
Incorporated sequences were modified: two mutations in the gene aroA (P101S, T971) and three mutations in the gene thiO (G51S, A54R, H244R).
LMO characteristics
Modified traits
Common use(s)
  • Feed
  • Biofuel