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Modified Organism
Potato modified for pathogen resistance
Record information and status
Record ID
108134
Status
Published
Date of creation
2015-06-02 16:41 UTC (g.mozgova@igc.by)
Date of publication
2015-06-23 16:34 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Potato modified for pathogen resistance
Transformation event
Solanum tuberosum L, variety Scarb N
Developer(s)
Анатолий Николаевич Евтушенков
Head of the Department of Molecular Biology
Department of Molecular Biology
Faculty of Biology of the Belarusian State University
Kurchatova, 10
Minsk
Belarus
Phone:+375 17 2095898
Email:evtushenkov@bsu.by
Description
Intracellular expression of the transgenic hrpN gene has been shown to upregulate the expression of endogenous PR2, PR5 and PR10 genes which may confer increased protection against biotrophic pathogens in transgenic Solanum tuberosum L. plants as compared to control.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Variety: Scarb N
Characteristics of the transformation process
Vector
pBIP-N constructed on the basis of pBI121 binary vector by insertion of hrpN-FLAG sequence into T-region
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Harpin protein coding sequence - Pectobacterium carotovorum - PECCA
Resistance to diseases and pests
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
Notes regarding the genetic elements introduced or modified in this LMO
The hrpN sequence is modified at the 3 'end by addition of a short (8 codons) FLAG-epitope, allowing easy detection of its product by means of commercial anti-FLAG antibodies.
LMO characteristics
Modified traits
  • Resistance to diseases and pests
Common use(s)
  • Research