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Modified Organism
Barley modified for tolerance to drought and nutrient deficiencies
Record information and status
Record ID
108708
Status
Published
Date of creation
2015-09-02 19:16 UTC (hana.jirakova@gmail.com)
Date of last update
2015-09-10 22:13 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-09-10 22:13 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Barley modified for tolerance to drought and nutrient deficiencies
Transformation event
pEXP:CKX2
Developer(s)
Palacky University in Olomouc
Faculty of Science
Krizkovskeho 8
Olomouc
Czech Republic, 771 47
Phone:+420 585 634 060
Fax:+420 585 634 002
Email:dekanat.prf@upol.cz
Url:Facuty of Science, the Palacky University in Olomouc
Description
The pEXP:CKX2  barley line has been genetically modified by inserting the Arabidopsis thaliana cytokinin dehydrogenase 2 gene under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice

Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, which are chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants. An important trait regulated by cytokinin is the size of the root system.

A reduced cytokinin status in plants (including barley) causes an enhanced root system which might render plants more tolerant to drought and nutrient deficiencies in the soil. Crop yield is often limited by the availability of water and soil-derived mineral nutrients. A larger root system may enable plants to gain access to more water and nutrients and in this way to cope with adverse environmental conditions.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Related LMOs
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
pEXP:CKX2
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
LOC_Os04g11040.1 promoter - Oryza sativa - Rice, ORYSA
Cytokinin dehydrogenase 2 gene - Arabidopsis thaliana - Thale cress, Mouse-ear cress, Arabidopsis, ARATH
Tolerance to abiotic stress - Drought
Ubiquitin gene promoter - Zea mays - Maize, Corn, MAIZE
Hygromycin B phosphotransferase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Hygromycin
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
The transgenic line shows an up to 8-fold higher CKX enzyme activity in roots than the wild type. In contrast, CKX enzyme activity is similar to the control plants in leaves.

The cytokinin dehydrogenase 2 gene is under the control of the root-specific promoter derived from the expressed protein gene LOC_Os04g11040.1 of rice.

The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays polyubiquitin gene.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism3 records
Risk Assessment1 record