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Modified Organism
Barley modified for tolerance to drought and nutrient deficiencies
Record information and status
Record ID
108710
Status
Published
Date of creation
2015-09-02 19:16 UTC (hana.jirakova@gmail.com)
Date of publication
2015-09-10 22:12 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Barley modified for tolerance to drought and nutrient deficiencies
Transformation event
CKX1:RNAi
Developer(s)
Palacky University in Olomouc
Faculty of Science
Krizkovskeho 8
Olomouc
Czech Republic, 771 47
Phone:+420 585 634 060
Fax:+420 585 634 002
Email:dekanat.prf@upol.cz
Url:Facuty of Science, the Palacky University in Olomouc
Description
The CKX1:RNAi barley line has been genetically modified by inserting the a copy of the native Hordeum vulgare cytokinin dehydrogenase 1 gene under the control of the constitutive ubiquitin gene promoter from maize.

Cytokinin dehydrogenases catalyze the irreversible degradation of cytokinins in a single enzymatic step by oxidative side chain cleavage. Cytokinins, chemically N6-substituted purine derivatives, are a class of plant hormones that regulate cell division as well as a large number of developmental events in plants.

Beside the others, cytokinins influence grain filling by regulation of the cell-wall invertase, the important enzyme in a polysacharides metabolism.

Increased cytokinin levels in the grains might enhance the process of grain filling and lead to the production of bigger grains. As the HvCKX1 gene is naturally expressed in the young barley roots and grains, the constitutive expression should not influence the other parts of the plant.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Point of collection or acquisition of the recipient organism
cv. Golden Promise
Related LMOs
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Barley modified for tolerance to drought and nutrient deficiencies
Resistance to antibiotics - Hygromycin Selectable marker genes and reporter genes Tolerance to abiotic stress - Drought
Characteristics of the transformation process
Vector
CKX1:RNAi
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Cytokinin dehydrogenase 1 gene - Hordeum vulgare - Barley, HORVU
Tolerance to abiotic stress - Drought
Ubiquitin gene promoter - Zea mays - Maize, Corn, MAIZE
Hygromycin B phosphotransferase gene - Escherichia coli - ECOLX
Resistance to antibiotics - Hygromycin
Selectable marker genes and reporter genes
Notes regarding the genetic elements introduced or modified in this LMO
The transgenic line showed an up 4-fold lower CKX activity in the barley grains compared to the wild type plants. The total yield from CKX1:RNAi barley was 20% higher than the yield of wild type barley.

The cytokinin dehydrogenase 1 silencing cassette is under the control of the constitutive ubiquitin gene promoter from maize.

The Hygromycin B phosphotransferase selection marker is under the control of the promoter region from Zea mays ubiquitin gene.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes

Records referencing this document (4)
IDDescription
4record(s) found
Modified Organism3 records
Risk Assessment1 record