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Modified Organism
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.
Record information and status
Record ID
108713
Status
Published
Date of creation
2015-08-25 09:36 UTC (german_bch@bvl.bund.de)
Date of last update
2016-07-04 10:49 UTC (german_bch@bvl.bund.de)
Date of publication
2016-07-15 16:48 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Black Nightshade with reduced pathogenesis-related protein 1S synthesis.
Transformation event
S03/140-5
Developer(s)
Max-Planck-Institut für Chemische Ökologie
Hans-Knöll-Strasse 8
D-07745 Jena
Germany
Description
In the transgenic plants of the line S03/140-5 the synthesis of the plant's pathogenesis-related (PR) protein 1S is to be reduced by post transcriptional gene silencing.

The pr-1S gene derived from Solanum nigrum codes for a PR protein. PR proteins are encoded by the host plant in response to pathogen attacks or comparable stress factors.

For this purpose, a construct was developed in which two complementary internal fragments of the pr-1S gene derived from Solanum nigrum were arranged in sense and antisense orientation, separated by a spacer. As a result of genetic modification, the resistance of the transgenic plant to phytopathogenic microorganisms is reduced by targeted reduction of the formation of the PR-1S protein.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum nigrum - Black nightshade, SOLNI
Related LMOs
Black Nightshade with reduced pathogenesis-related protein 1S synthesis
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Black nightshade modified for reduced production of endogenous proteinase inhibitors
Resistance to antibiotics - Hygromycin Resistance to diseases and pests - Insects Selectable marker genes and reporter genes
Black nightshade modified for reduced production of endogenous prosystemin
Resistance to antibiotics - Hygromycin Resistance to diseases and pests Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pSOL3PRIS
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Pathogenesis-related protein 1S gene
0.30 Kb
 
 
Pyruvate orthophosphate dikinase, Intron 3
0.79 Kb
 
 
Pathogenesis-related protein 1S gene
0.30 Kb
 
 
CaMV 35S terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The plasmid pSOL3PRIS used for transformation includes a construct, in which two complementary 295 bp long internal fragments of the pr-1S gene derived from Solanum nigrum were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia, of which splicing activity is suspected, was used as a spacer. Expression is controlled by the promoter and the terminator of the 35S gene of the cauliflower mosaic virus (CaMV).

The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and terminator of the nopaline synthase gene of Agrobacterium tumefaciens was used as selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
LMO characteristics
Modified traits
  • Resistance to diseases and pests
Common use(s)
  • Research

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication1 record
Modified Organism3 records
Risk Assessment1 record