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Modified Organism
MON-87411-9 - Maize modified for herbicide tolerance and insect resistance
Record information and status
Record ID
108881
Status
Published
Date of creation
2015-09-30 04:16 UTC (Stacy.Scott@epa.govt.nz/15360)
Date of publication
2015-10-02 15:57 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for herbicide tolerance and insect resistance
Transformation event
MON 87411
Unique identifier
MON-87411-9
Developer(s)
Monsanto
Description
MON-87411-9 maize was modified to contain a suppression cassette that expresses an inverted repeat sequence designed to match the sequence of western corn rootworm (WCR).

The expression of the suppression cassette results in the formation of a double-stranded RNA (dsRNA) transcript containing a 240 bp fragment of the WCR Snf7 gene (DvSnf7). Upon consumption, the plant-produced dsRNA in MON 87411 is recognized by the CRW's RNA interference (RNAi) machinery resulting in down regulation of the targeted DvSnf7 gene leading to CRW mortality.

MON 87411 also contains a cry3Bb1 gene that produces a modified Bacillus thuringiensis (subsp. kumamotoensis) Cry3Bb1 protein to protect against CRW larval feeding.

In addition, MON 87411 contains the cp4 epsps gene from Agrobacterium sp. strain CP4 that encodes for the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein, which confers tolerance to glyphosate
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PV-ZMIR10871
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Snf7 coding sequence
0.24 Kb
 
 
Snf7 coding sequence
0.24 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
pIIG gene promoter
0.95 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.48 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.21 Kb
 
 
Alpha Tubulin Gene promoter
2.18 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
Alpha Tubulin Gene terminator
0.58 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The DvSnf7p sequence is the partial coding sequence of the Snf7 gene from Diabrotica virgifera virgifera encoding the SNF7 subunit of the ESCRT-III complex. The DvSnf7 suppression cassette contains two 240 bp DvSnf7p sequences in an inverted orientation. There is an intervening sequence of 150 nucleotides between the two DvSnf7p sequences. When the suppression cassette is transcribed, the RNA expressed forms a hairpin loop thereby allowing the formation of double stranded DvSnf7 RNA. The DvSnf7p sequences in the suppression cassette produce a 240 bp dsRNA that upon transcription triggers the RNAi mechanism.

Sequencing, PCR, and bioinformatic analysis indicated that a single, intact insert of the DvSnf7 suppression cassette and the cry3Bb1 and cp4 epsps expression cassettes were stably integrated into the maize genome. Analyses also indicated the absence of the plasmid backbone DNA in MON 87411 maize.
LMO characteristics
Modified traits

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication2 records
Modified Organism1 record
Risk Assessment2 records