| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
MON-887Ø1-3 x MON-88913-8 x MON-15985-7 - Insect resistant, Herbicide tolerant Cotton
Record information and status
Record ID
108887
Status
Published
Date of creation
2015-10-05 15:13 UTC (dina.abdelhakim@cbd.int)
Date of last update
2017-02-13 17:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2017-02-13 17:48 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant, Herbicide tolerant Cotton
Transformation event
MON88701 x MON88913 x MON15985
Unique identifier
MON-887Ø1-3 x MON-88913-8 x MON-15985-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked cotton line MON88701 x MON88913 x MON15985 expresses several novel proteins: the delta-endotoxins the Cry1Ac and Cry2Ab which confer resistance to lepidopteran pests, EPSPS (5-enolpyruvylshikimaete-3-phosphate synthase) that confers tolerance to the herbicide glyphosate, as well as Dicamba monooxygenase gene that confers tolerance to the herbicide dicamba and Phosphinothricin N-acetyltransferase gene that confers tolerance to the herbicide glufosate.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
MON-887Ø1-3 - Dicamba and Glufosinate Tolerant Cotton
Resistance to herbicides - Glufosinate
MON-88913-8 - Roundup Ready™ Flex™ cotton
Resistance to herbicides - Glyphosate
Show detection method(s)
MON-15985-7 - Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Selectable marker genes and reporter genes
Show detection method(s)
Gossypium hirsutum - Cotton
Related LMOs
MON-88913-8 x MON-15985-7 - Roundup Ready™ Flex™ Bollgard II™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHHT6997, PV-GHGT35, PV-GHBK04 and PV-GHBK11
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.00 Kb
 
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
HSP 70 5' untranslated leader sequence
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
Cry2Ab2
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S Enhancer
0.00 Kb
 
 
Actin 8 Promoter
0.00 Kb
 
 
Actin 8 Leader sequence
0.00 Kb
 
 
Actin 8 Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
FMV 35S Enhancer
0.00 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.00 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
 
PC1SV Promoter
0.43 Kb
 
 
TEV 5' Untranslated Region
0.13 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
Dicamba monooxygenase gene
1.02 Kb
 
 
E6 gene terminator
0.31 Kb
 
 
CaMV 35S promoter
0.61 Kb
 
 
HSP 70 5' untranslated leader sequence
0.10 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A stacked insect-resistant and herbicide-tolerant cotton derived by crossing MON-88913-8 with MON-15985-7 and MON-887Ø1-3, includes the cry1Ac gene and the cry2Ab gene from Bacillus thuringiensis subsp. kurstaki conferring resistance to lepidopteran pests, and the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase that confers tolerance to the herbicide glyphosate as well as Dicamba monooxygenase gene that confers tolerance to the herbicide dicamba and Phosphinothricin N-acetyltransferase gene that confers tolerance to the herbicide glufosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin and was used as a selectable marker.

DNA insert from MON 88913 vector PV-GHGT35
Roundup Ready® Flex cotton (MON 88913) was developed to allow the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option in cotton production. This genetically engineered cotton contains a novel form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows MON 88913 to survive otherwise lethal applications of glyphosate. The EPSPS gene introduced into MON 88913 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens strain CP4; the EPSPS enzyme expressed by this gene is tolerant to glyphosate. MON 88913 cotton contains two copies of the EPSPS gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage.

DNA insert from MON15985 vector PV-GHBK11 and PV-GHBK04
Event 15985 (tradename Bollgard II®) was derived from the retransformation of transgenic cotton line MON 531. As a result of these two transformation events, MON15985 contains the cry1Ac gene and the cry2Ab conferring resistance to lepidopteran pests as well as copies of the nptII and uidA and aadA genes.

DNA insert from MON88701 vector PV-GHHT6997
Cotton modified with the insertion of transformation cassette containing the Dicamba monooxygenase (DMO) gene and Bialaphos resistance (BAR) gene to confer resistance to the herbicides Dicamba and Glufosinate respectively.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Resistance to Dicamba
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Risk Assessment2 records