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Gene and DNA Sequence
Record information and status
Record ID
108898
Status
Published
Date of creation
2015-09-30 13:17 UTC (german_bch@bvl.bund.de)
Date of publication
2015-10-06 21:13 UTC (dina.abdelhakim@cbd.int)

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General information
Name of the gene or DNA sequence
Beta-1,3-1,4-glucanase gene
Alternate Genetic Element Name (synonyms)
Endo-1,3(4)-b-glucanase
Abbreviation
CS-B-1,3(4)-glucanase-SYNTH
Category of DNA sequence
  • Protein coding sequence
Is the gene / DNA sequence is a synthetic molecule?
Yes
Characteristics of the protein coding sequence
Name of the protein expressed by the coding sequence
Beta-1,3-1,4-glucanase
Biological function
This thermostable Beta-1,3-1,4-glucanase is the chimeric product resulting from the recombination of two Beta-1,3-1,4-glucanase from Bacillus amyloliquefaciens and Bacillus macerans.

The resulting protein depolymerises glucans in the aleurone layer and the endosperm during caryopsis germination.
Related trait(s) or use(s) in biotechnology
Additional Information
Additional Information
The gene introduced into barley was cloned by intragenic recombination of two (1,3-1,4)-β-glucanases from Bacillus amyloliquefaciens and Bacillus macerans. The enzyme is composed of amino acids 1-12 of the glucanase from Bacillus amyloliquefaciens and amino acids 13-214 of glucanase from Bacillus macerans. Tyrosin at position 13 was deleted. The nucleotide sequence was codon-optimized to ensure functionality of the microbial gene in plants by raising the GC content to 63%.

Records referencing this document (3)
IDDescription
3record(s) found
Country's Decision or any other Communication1 record
Modified Organism1 record
Risk Assessment1 record