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Modified Organism
Barley modified for increased (1,3-1,4)-β-glucan degradation
Record information and status
Record ID
108902
Status
Published
Date of creation
2015-09-30 13:17 UTC (german_bch@bvl.bund.de)
Date of publication
2015-10-08 17:33 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Barley modified for increased (1,3-1,4)-β-glucan degradation
Transformation event
pJH271-Beta-Glu-307
Developer(s)
Justus-Liebig-Universität Gießen
Institut für Phytopathologie und Angewandte Zoologie
Justus-Liebig-Universität Gießen
Heinrich-Buff-Ring 26-32
Gießen, Hessen
Germany, 35392
Phone:+ 49 641 99-37490
Fax:+ 49 641 99-37499
Email:info@hrz.uni-giessen.de,Karl-Heinz.Kogel@agrar.uni-giessen.de,Andreas.Vilcinskas@agrar.uni-giessen.de
Url:http://www.uni-giessen.de/ipaz-alt/home.htm
Description
Barley was modified with the insertion of a chimeric (1,3-1,4)-β-glucanase leading to increased degradation of (1,3-1,4)-β-glucane in the endosperm and aleuron during germination.

This allows α-amylases and proteases to access starch and storage proteins that are stored in the endosperm, and therefore providing carbohydrates and amino acids to the growing shoot. This increases the nutritional profile of this barley when used as feed. For usage in beer brewing, where barley grains are heated, the expressed (1,3-1,4)-β-glucanase is thermostable.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Characteristics of the transformation process
Vector
pJH271
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
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Ubiquitin Intron 1
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Phosphinothricin N-acetyltransferase gene
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Nopaline Synthase Gene Terminator
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CaMV 35S promoter
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Green Fluorescent Protein gene
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Nopaline Synthase Gene Terminator
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Nopaline Synthase Gene Terminator
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Beta-1,3-1,4-glucanase gene
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Hor3-1 transit peptide
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Hor3-1 gene promoter
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
The chimeric (1,3-1,4)-β-glucanase gene was synthesised through the intragenic recombination of two (1,3-1,4)-β-glucanases, specifically amino acids 1-12 of the glucanaseBacillus amyloliquefaciens and amino acids 13-214 of glucanase from Bacillus macerans.

The nucleotide sequence was codon-optimized to ensure functionality of the microbial gene in plants by raising the GC content to 63%. The resulting enzyme has the same substrate specificity as it homologous barley counterpart but has enhanced thermostability.

Te expression cassetes were cloned in to the pBIN19 vector and also includes LB and RB of Agrobacterium tumefaciens, parts of the lacZ gene from E. coli and fragments of the bacteriophage M13 origin of replication, which are not functional in plants, as well as fragments of the nopaline synthase gene promoter from Agrobacterium tumefaciens.

The expression of green fluorescent protein (GFP) and bialaphos resistance gene (bar) serve as selection markers
LMO characteristics
Modified traits
Common use(s)
  • Research
Additional Information
Additional Information
For transformation of pJH271-Beta-Glu-307 the barley variety Golden promise was used. By crossing with the elite line Baronesse for several generations the genetic background changed, however, a few alleles from Golden Promise are reported to be introgressed into the genome.

Records referencing this document (2)
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2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record