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Modified Organism
Barley modified for increased fungal resistance
Record information and status
Record ID
108905
Status
Published
Date of creation
2015-09-30 13:20 UTC (german_bch@bvl.bund.de)
Date of publication
2015-10-08 21:43 UTC (dina.abdelhakim@cbd.int)

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Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Barley modified for increased fungal resistance
Transformation event
pYW210-9-(4001-4360)
Developer(s)
Justus-Liebig-Universität Gießen
Institut für Phytopathologie und Angewandte Zoologie
Justus-Liebig-Universität Gießen
Heinrich-Buff-Ring 26-32
Gießen, Hessen
Germany, 35392
Phone:+ 49 641 99-37490
Fax:+ 49 641 99-37499
Email:info@hrz.uni-giessen.de,Karl-Heinz.Kogel@agrar.uni-giessen.de,Andreas.Vilcinskas@agrar.uni-giessen.de
Url:http://www.uni-giessen.de/ipaz-alt/home.htm
Description
Barley was modified with the insertion of a synthetic form of endochitinase 42 from Trichoderma harzianum with the aim of increasing resistance to root rot caused by Rhizoctonia species through the hydrolyses of  N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrinsis, which are major structural components of the Rhizoctonia species cell wall.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hordeum vulgare - Barley, HORVU
Point of collection or acquisition of the recipient organism
Variety: Golden Promise
Characteristics of the transformation process
Vector
pYW210
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
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Ubiquitin Intron 1
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Chitinase 33 transit peptide
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Endochitinase 42 coding sequence
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Nopaline Synthase Gene Terminator
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Ubiquitin gene promoter
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Ubiquitin Intron 1
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Phosphinothricin N-acetyltransferase gene
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Nopaline Synthase Gene Terminator
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Further details
Notes regarding the genetic elements introduced or modified in this LMO
Two gene cassettes are integrated into the transgenic barley line pYW210-9-(4001-4360).

Within the first cassette the expression of the endochitinase gene cThEn42(GC) is regulated by the ubi-1 promoter  together with an exon and an intron of the ubi-1 gene (ubiquitin gene) from Zea mays (maize) and the nopaline synthase gene terminator T-nos from Agrobacterium tumefaciens. Secretion of the synthesized protein to the apoplast is allowed by the signal peptide of HvChi33 (33 kDa endochitinase from Hordeum vulgare L. (barley)) that is located upstream of cThEn42(GC) in the gene cassette. The nucleotide sequence of cThEn42 from the donor organism Trichoderma harzianum was codon optimized (65% GC content) to ensure the expression of the fungal gene in barley.

The second gene cassette comprises the P-ubiZM1, the phosphinothricin acetyltransferase gene from Streptomyces hygroscopicus and T-nos and is used as a selection marker.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research
Additional Information
Additional Information
Based on transcriptome and metabolome analysis Kogel et al. (2010) reported no statistically significant differences in resistance towards root rot between the transgenic plant and its wild-type counterpart.

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record