| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
Rubber tree modified to express a reporter gene
Record information and status
Record ID
108906
Status
Published
Date of creation
2015-09-18 09:38 UTC (anita@nre.gov.my)
Date of publication
2015-10-09 12:51 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rubber tree modified to express a reporter gene
Transformation event
HT1
Developer(s)
Dr. Sunderasan Elumalai
Research Officer
Genetic Transformation and Tissue Culture Programme, Production Advancement Division
Malaysian Rubber Board (LGM)
Genetic Transformation and Tissue Culture Programme,
Production Advancement Division,
Malaysian Rubber Board,

Sungai Buloh, Selangor
Malaysia, 47000
Phone:+603-61459599
Fax:+603-61565251
Email:sunderasan@lgm.gov.my
Description
Hevea brasiliensis was modified to express the glucuronidase (GUS) reporter gene encoding glucuronidase enzyme that hydrolyzes x-gluc - a colourless substrate, into soluble blue coloured product. This serves as an indicator of successful expression of transgene product in the target tissue.

GUS enzyme activity has been detected in latex and leaf tissue of transformed Hevea plants.

Hevea transformed with GUS also harbours the nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hevea brasiliensis - Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR
Point of collection or acquisition of the recipient organism
Clone GL1
Characteristics of the transformation process
Vector
p35S-GUS-INT
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Beta-Glucuronidase coding sequence
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Agrobacterium tumefaciens containing p35S-GUS-INT plant expression vector was a gift from Prof. L. Willmitzer, Institut fur Genbiologische Forschung, Berlin.

The inserted genetic material is the region between the left border and right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Research

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record