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Modified Organism
Rubber tree modified to express the scFv4715 antibody fragment
Record information and status
Record ID
108912
Status
Published
Date of creation
2015-09-18 09:48 UTC (anita@nre.gov.my)
Date of publication
2015-10-09 18:40 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rubber tree modified to express the scFv4715 antibody fragment
Transformation event
HT2
Developer(s)
Dr. Sunderasan Elumalai
Research Officer
Genetic Transformation and Tissue Culture Programme, Production Advancement Division
Malaysian Rubber Board (LGM)
Genetic Transformation and Tissue Culture Programme,
Production Advancement Division,
Malaysian Rubber Board,

Sungai Buloh, Selangor
Malaysia, 47000
Phone:+603-61459599
Fax:+603-61565251
Email:sunderasan@lgm.gov.my
Description
Hevea brasiliensis was modified to express the scFv4715 recombinant antibody, which is intended to bind to S. gordonii coat protein, the causal agent of dental plaque/cavity, with the aim to prevent tooth decay. Expression of the scFv4715 gene in Hevea brasiliensis allows for the commercial production of this protein for pharmaceutical purposes.

Hevea with scFv4715 also harbours nptII gene that confers resistance to kanamycin, which was incorporated in the growth media to select transformed callus after co-cultivation with Agrobacterium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Hevea brasiliensis - Rubber tree, Para rubber tree, SiringaTree, Jebe, HEVBR
Point of collection or acquisition of the recipient organism
Clone GL1
Characteristics of the transformation process
Vector
pGPTV-Kan
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
Pathogenesis-related protein 1a signal peptide
0.00 Kb
 
 
Coding sequence of scFv4715 antibody
0.00 Kb
 
 
FLAG Tag
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Transcript 7 gene 3' untranslated region
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The inserted genetic material is the region between the left boarder and the right boarder (T-DNA) from the Ti plasmid of Agrobacterium tumefaciens harbouring the above described gene construct.
LMO characteristics
Modified traits
Common use(s)
  • Pharmaceutical
  • Research
Additional Information
Other relevant website address or attached documents

Records referencing this document (2)
IDDescription
2record(s) found
Country's Decision or any other Communication1 record
Risk Assessment1 record