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Modified Organism
MON-ØØ179-5 x MON-ØØ1Ø1-8 - Herbicide tolerant, reduced ligning alfalfa
Record information and status
Record ID
109024
Status
Published
Date of creation
2015-10-15 16:22 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-15 16:22 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide tolerant, reduced ligning alfalfa
Transformation event
KK179 x J101
Unique identifier
MON-ØØ179-5 x MON-ØØ1Ø1-8
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The stacked alfalfa line MON-ØØ179-5 x MON-ØØ1Ø1-8 was obtained through the traditional cross breading of each of the parental organisms to produce alfalfa that expresses the EPSPS gene and a caffeoyl CoA 3-O-methyltransferase (CCOMT) suppression cassette. The expression of these genetic elements are expected to confer tolerance to the glyphosate herbicide and reduced levels of guaiacyl lignin, respectively.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Medicago sativa - Alfalfa, Lucerne, MEDSV
MON-ØØ1Ø1-8 - Roundup Ready™ alfalfa
Resistance to herbicides - Glyphosate
MON-ØØ179-5 - Reduced Lignin Alfalfa
Changes in quality and/or metabolite content - Lignin Resistance to antibiotics - Kanamycin
Characteristics of the transformation process
Vector
PV-MSPQ12633 and PV-MSHT4
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Pal2 gene Promoter
0.00 Kb
 
 
Caffeoyl CoA 3-O-methyltransferase gene
0.52 Kb
 
 
Caffeoyl CoA 3-O-methyltransferase gene
0.52 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
FMV 35S promoter
0.00 Kb
 
 
HSP 70 5' untranslated leader sequence
0.00 Kb
 
 
Chloroplast transit peptide 2
0.00 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
0.00 Kb
 
 
rbcS-E9 gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from KK179 vector PV-MSPQ12633
KK179 was developed to have reduced levels of guaiacyl lignin (G). This reduction in G lignin leads to reduced accumulation of total lignin in alfalfa through the suppression of caffeoyl CoA 3-O-methyltransferase (CCOMT), a key enzyme in the lignin biosynthetic pathway through RNAi gene suppression.
The PV-MSPQ12633  vector also contains a transformation cassette expressing the nptII.

DNA insert from J101 vector PV-MSHT4
J101 contains the epsps gene from Agrobacterium tumefaciens strain CP4 to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Risk Assessment3 records